Arabzadeh Naser, Khavari-Nejad Ramazan Ali
Agricultural and Natural Resources Researches Center, Sodoughi Avenue, P.O. Box 76175-538, Kerman, Iran.
Department of Biology, Tarbiat Moallem University, Tehran, Iran.
Pak J Biol Sci. 2013 Apr 15;16(8):351-61. doi: 10.3923/pjbs.2013.351.361.
Superoxide dismutase activity changes were studied at different periodic tensions using of spectrophotometric measurement of decline in NitroBlue Tetrazolium reduction to Blue Formazan at 560 nm in Haloxylon aphyllum and Haloxylon persicum. The aim of this study was to investigate the changes of superoxide dismutase in applied drought stress in two species of Haloxylon. The results showed that the effect of drought stress on increase in superoxide dismutase activity was significant (p < 0.01) in two haloxylon species. Drought increased enzyme activity at severe tensions. When two haloxylon species were placed under 7 and 14 days no-watering treatments (mild tensions), the enzyme activity was more than its activity in control treatment and less than one in 21 and 28 days no-watering treatments (severe tensions). The enzyme activity in branchlets of Haloxylon aphyllum under 21 and 28 days no-watering treatments was 20.2 and 29.5% more than its activity under control treatment respectively. This activity in Haloxylon persicum was 21.6 and 31.4% more than its activity under control treatment, respectively. With the advent of drought, superoxide dismutase activity increased in two species of haloxylon. The increasing of superoxide dismutase activity in time of dryness advent in Haloxylon aphyllum was more than Haloxylon persicum, which can be raised as an acceptable factor and vindicator in being more resistant of Haloxylon aphyllum to environmental drought.
采用分光光度法测定无叶假木贼和波斯假木贼在560 nm处氮蓝四唑还原为蓝色甲臜的吸光度下降情况,研究了不同周期胁迫下超氧化物歧化酶(SOD)活性的变化。本研究旨在探讨两种假木贼在干旱胁迫下超氧化物歧化酶的变化。结果表明,干旱胁迫对两种假木贼超氧化物歧化酶活性增加的影响显著(p < 0.01)。干旱在重度胁迫下会增加酶活性。当两种假木贼分别进行7天和14天的不浇水处理(轻度胁迫)时,酶活性高于对照处理,而低于21天和28天不浇水处理(重度胁迫)时的酶活性。在21天和28天不浇水处理下,无叶假木贼小枝中的酶活性分别比对照处理高20.2%和29.5%。波斯假木贼的这一活性分别比对照处理高21.6%和31.4%。随着干旱的出现,两种假木贼的超氧化物歧化酶活性均增加。干旱出现时,无叶假木贼超氧化物歧化酶活性的增加幅度大于波斯假木贼,这可作为无叶假木贼对环境干旱更具抗性的一个可接受因素和证据。