State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China.
College of Agriculture and Forestry, Longdong University, Qingyang 745000, China.
Int J Mol Sci. 2017 Dec 29;19(1):84. doi: 10.3390/ijms19010084.
, a perennial xero-halophyte, is an essential species for investigating the effects of drought on desert tree. To gain a comprehensive knowledge on the responses of to drought stress, we specially performed the molecular and physiological analysis of in response to -0.75 MPa osmotic stress for six and 24 h in lab condition via RNA-seq and digital gene expression (DGE). In total, 87,109 unigenes with a mean length of 680 bp and 13,486 potential simple sequence repeats (SSRs) were generated, and 3353 differentially expressed genes (DEGs) in shoots and 4564 in roots were identified under stress. These DEGs were mainly related to ion transporters, signal transduction, ROS-scavenging, photosynthesis, cell wall organization, membrane stabilization and hormones. Moreover, the physiological changes of inorganic ions and organic solute content, peroxidase (POD) activity and osmotic potential were in accordance with dynamic transcript profiles of the relevant genes. In this study, a detailed investigation of the pathways and candidate genes identified promote the research on the molecular mechanisms of abiotic stress tolerance in the xero-halophytic species. Our data provides valuable genetic resources for future improvement of forage and crop species for better adaptation to abiotic stresses.
沙冬青是一种多年生的旱生盐生植物，是研究干旱对沙漠树木影响的重要物种。为了全面了解 对干旱胁迫的响应，我们特别通过 RNA-seq 和数字基因表达（DGE），在实验室条件下对 进行了为期 6 和 24 小时、-0.75 MPa 渗透胁迫的分子和生理分析。总共生成了 87109 条具有 680bp 平均长度和 13486 个潜在简单重复序列（SSR）的 unigenes，并且在胁迫下，在地上部鉴定出 3353 个差异表达基因（DEG），在根部鉴定出 4564 个。这些 DEG 主要与离子转运体、信号转导、ROS 清除、光合作用、细胞壁组织、膜稳定和激素有关。此外，无机离子和有机溶质含量、过氧化物酶（POD）活性和渗透势的生理变化与相关基因的动态转录谱一致。在这项研究中，对途径和候选基因的详细研究促进了对旱生盐生植物非生物胁迫耐受性的分子机制的研究。我们的数据为未来改良饲料和作物品种以更好地适应非生物胁迫提供了有价值的遗传资源。
