Yang Fang, Lv Guanghui
School of Ecology and Environment, Xinjiang University, Urumqi, China.
Key Laboratory of Oasis Ecology, Ministry of Education, Urumqi, China.
Front Plant Sci. 2022 Oct 17;13:1020367. doi: 10.3389/fpls.2022.1020367. eCollection 2022.
and , as typical desert plants, show strong drought tolerance and environmental adaptability. They are ideal model plants for studying the molecular mechanisms of drought tolerance. Transcriptomic and metabolomic analyses were performed to reveal the response mechanisms of and to a drought environment at the levels of transcription and physiological metabolism. The results showed that the morphological structures of and showed adaptability to drought stress. Under drought conditions, the peroxidase activity, abscisic acid content, auxin content, and gibberellin content of increased, while the contents of proline and malondialdehyde decreased. The amino acid content of was increased, while the contents of proline, malondialdehyde, auxin, and gibberellin were decreased. Under drought conditions, 12,233 and 17,953 differentially expressed genes (DEGs) were identified in and , respectively, including members of multiple transcription factor families such as FAR1, AP2/ERF, C2H2, bHLH, MYB, C2C2, and WRKY that were significantly up-regulated under drought stress. In the positive ion mode, 296 and 452 differential metabolites (DEMs) were identified in and , respectively; in the negative ion mode, 252 and 354 DEMs were identified, primarily in carbohydrate and lipid metabolism. A combined transcriptome and metabolome analysis showed that drought stress promoted the glycolysis/gluconeogenesis pathways of and and increased the expression of amino acid synthesis pathways, consistent with the physiological results. In addition, transcriptome and metabolome were jointly used to analyze the expression changes of the genes/metabolites of and that were associated with drought tolerance but were regulated differently in the two plants. This study identified drought-tolerance genes and metabolites in and and has provided new ideas for studying the drought stress response of .
并且，作为典型的沙漠植物，表现出很强的耐旱性和环境适应性。它们是研究耐旱分子机制的理想模式植物。进行了转录组学和代谢组学分析，以揭示其在转录和生理代谢水平上对干旱环境的响应机制。结果表明，其形态结构表现出对干旱胁迫的适应性。在干旱条件下，某植物的过氧化物酶活性、脱落酸含量、生长素含量和赤霉素含量增加，而脯氨酸和丙二醛含量降低。另一植物的氨基酸含量增加，而脯氨酸、丙二醛、生长素和赤霉素含量降低。在干旱条件下，分别在某植物和另一植物中鉴定出12233个和17953个差异表达基因（DEG），包括FAR1、AP2/ERF、C2H2、bHLH、MYB、C2C2和WRKY等多个转录因子家族的成员，这些成员在干旱胁迫下显著上调。在正离子模式下，分别在某植物和另一植物中鉴定出296个和452个差异代谢物（DEM）；在负离子模式下，鉴定出252个和354个DEM，主要涉及碳水化合物和脂质代谢。转录组和代谢组联合分析表明，干旱胁迫促进了某植物和另一植物的糖酵解/糖异生途径，并增加了氨基酸合成途径的表达，这与生理结果一致。此外，转录组和代谢组联合用于分析某植物和另一植物中与耐旱性相关但在两种植物中调控方式不同的基因/代谢物的表达变化。本研究鉴定了某植物和另一植物中的耐旱基因和代谢物，并为研究其干旱胁迫响应提供了新思路。
