School of Ecology and Environment, Xinjiang University, Urumqi 830017, China.
Key Laboratory of Oasis Ecology, Ministry of Education, Urumqi 830017, China.
Int J Mol Sci. 2023 May 22;24(10):9099. doi: 10.3390/ijms24109099.
and , as typical desert plants in arid areas, show strong drought tolerance and environmental adaptability and are therefore ideal model plants for studying the molecular mechanisms of drought tolerance. A metabolomic analysis of and in their natural environment is lacking, and their metabolic response to drought therefore remains unclear. To elucidate the response of and to drought at the metabolic level, a non-targeted metabolomics analysis was carried out herein. Under a dry environment, exhibited 296 and 252 differentially expressed metabolites (DEMs) in the positive and negative ion modes, respectively, whereas 452 and 354 DEMs were identified in the positive and negative ion modes in , respectively. The results indicated that responds to drought by increasing the content of organic nitrogen compounds and lignans, neolignans, and related compounds, and reducing the content of alkaloids and derivatives. By contrast, adapts to the dry environment by increasing the content of organic acids and their derivatives and reducing the content of lignans, neolignans, and related compounds. In addition, and improved their osmoregulation ability, reactive oxygen species detoxification ability, and cell membrane stability by regulating the key metabolic pathways and anabolism of associated metabolites. This is the first metabolomics report on the response of and to drought in their natural environment, providing a foundation for the further study of their regulatory mechanisms under drought stress.
并且，作为干旱地区的典型沙漠植物，它们表现出很强的耐旱性和环境适应性，因此是研究耐旱性分子机制的理想模式植物。对 和 在其自然环境中的代谢组学分析尚缺乏研究，因此它们对干旱的代谢响应仍不清楚。为了阐明 和 在代谢水平上对干旱的响应，本文进行了非靶向代谢组学分析。在干燥环境下， 在正离子和负离子模式下分别显示出 296 和 252 个差异表达代谢物（DEMs），而 在正离子和负离子模式下分别鉴定出 452 和 354 个 DEMs。结果表明， 通过增加有机氮化合物和木脂素、新木脂素和相关化合物的含量，以及降低生物碱和衍生物的含量来响应干旱。相比之下， 通过增加有机酸及其衍生物的含量，降低木脂素、新木脂素和相关化合物的含量来适应干燥环境。此外， 和 通过调节相关代谢物的关键代谢途径和合成代谢来提高其渗透调节能力、活性氧解毒能力和细胞膜稳定性。这是关于 和 在其自然环境中对干旱响应的首次代谢组学报告，为进一步研究它们在干旱胁迫下的调控机制提供了基础。
