人日本血吸虫卵抗原特异性T细胞系。慢性日本血吸虫病患者体外辅助性和抑制性T细胞系及克隆的诱导。
Schistosoma japonicum egg antigen-specific T cell lines in man. Induction of helper and suppressor T cell lines and clones in vitro in a patient with chronic schistosomiasis japonica.
作者信息
Ohta N, Itagaki T, Minai M, Hirayama K, Hosaka Y
机构信息
Department of Parasitology, National Institute of Health, Tokyo, Japan.
出版信息
J Clin Invest. 1988 Mar;81(3):775-81. doi: 10.1172/JCI113383.
T cell lines (TCLs) specific for Schistosoma japonicum egg antigen were established from a patient with chronic schistosomiasis japonica to investigate the regulatory mechanism of S.japonicum egg antigen-driven T cell responses in man. All five TCLs tested were CD2+, CD4+, CD8-, and were strongly proliferative only to S. japonicum egg antigen in the absence of exogenous IL-2. All but one TCL produced IL-2-like lymphokines in vitro, indicating their helper T cell functions. One TCL, SjE-3, failed to produce IL-2-like lymphokines. Moreover, this TCL suppressed the specific proliferation of autologous peripheral blood lymphocytes to S. japonicum egg antigen. This TCL produced a soluble suppressor factor(s). These functional diversities among established TCLs were also confirmed by cloned T cells. Our observations might suggest that the regulatory system through helper and suppressor T-T interactions somehow involved in T cell responses to the egg antigen in human chronic schistosomiasis japonica.
为了研究日本血吸虫卵抗原驱动的人类T细胞反应的调节机制，从一名慢性日本血吸虫病患者体内建立了针对日本血吸虫卵抗原的T细胞系（TCLs）。所检测的所有五个TCLs均为CD2 +、CD4 +、CD8 -，并且在无外源性白细胞介素-2的情况下仅对日本血吸虫卵抗原有强烈增殖反应。除一个TCL外，所有TCL在体外均产生白细胞介素-2样淋巴因子，表明它们具有辅助性T细胞功能。一个TCL，即SjE-3，未能产生白细胞介素-2样淋巴因子。此外，该TCL抑制自体外周血淋巴细胞对日本血吸虫卵抗原的特异性增殖。该TCL产生了一种可溶性抑制因子。克隆的T细胞也证实了已建立的TCLs之间的这些功能差异。我们的观察结果可能表明，通过辅助性和抑制性T细胞相互作用的调节系统以某种方式参与了人类慢性日本血吸虫病中T细胞对卵抗原的反应。
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