Rabin H, Hopkins R F, Ruscetti F W, Neubauer R H, Brown R L, Kawakami T G
J Immunol. 1981 Nov;127(5):1852-6.
A continuous lymphoid cell line had been previously established from a gibbon with spontaneous lymphosarcoma. This cell line, designated as MLA144, when tested after several years in culture was shown to release spontaneously a factor biologically and biochemically similar to human T cell growth factor (TCGF). Conditioned media (CM) from MLA144 cells support growth and DNA synthesis of T cells from humans, several other species of primates, and also from mice and rabbits. The activity in the MLA144 CM is resistant to 60 degrees C and to low and high pH, has a m.w., as determined by gel filtration, of 21,500, elutes from DEAE-cellulose at 0.04 to 0.06 M sodium phosphate buffer, pH 7.6, and has an isoelectric point of about 6.45. Surface-marker analysis of MLA144 cells by rosetting techniques indicates that they are T cells lacking in the receptor for the Fc portion of IgG. The release of TCGF by MLA144 cells should have practical value in terms of ease of TCGF production and should be of great help in the facilitation of studies on the cell biology and molecular biology of TCGF production.
先前已从一只患有自发性淋巴肉瘤的长臂猿中建立了一个连续淋巴细胞系。这个细胞系被命名为MLA144，在培养数年之后进行检测时，发现它能自发释放一种在生物学和生物化学性质上与人类T细胞生长因子（TCGF）相似的因子。来自MLA144细胞的条件培养基（CM）能支持人类、其他几种灵长类动物以及小鼠和兔子的T细胞生长和DNA合成。MLA144 CM中的活性物质对60℃以及低pH和高pH均具有抗性，通过凝胶过滤测定其分子量为21,500，在pH 7.6的0.04至0.06 M磷酸钠缓冲液中从DEAE - 纤维素上洗脱，其等电点约为6.45。通过玫瑰花结技术对MLA144细胞进行表面标志物分析表明，它们是缺乏IgG Fc部分受体的T细胞。MLA144细胞释放TCGF在TCGF的生产便利性方面应具有实际价值，并且对促进TCGF生产的细胞生物学和分子生物学研究有很大帮助。
