Institute of Nanochemistry and Nanobiology, Shanghai University , Shanghai 200444, China.
ACS Appl Mater Interfaces. 2014 Feb 26;6(4):2791-8. doi: 10.1021/am4053317. Epub 2014 Feb 13.
New materials with good antibacterial activity and less toxicity to other species attract numerous research interest. Taking advantage of zinc oxide (ZnO) and graphene oxide (GO), the ZnO/GO composites were prepared by a facile one-pot reaction to achieve superior antibacterial properties without damaging other species. In the composites, ZnO nanoparticles (NPs), with a size of about 4 nm, homogeneously anchored onto GO sheets. The typical bacterium Escherichia coli and HeLa cell were used to evaluate the antibacterial activity and cytotoxicity of the ZnO/GO composites, respectively. The synergistic effects of GO and ZnO NPs led to the superior antibacterial activity of the composites. GO helped the dispersion of ZnO NPs, slowed the dissolution of ZnO, acted as the storage site for the dissolved zinc ions, and enabled the intimate contact of E. coli with ZnO NPs and zinc ions as well. The close contact enhanced the local zinc concentration pitting on the bacterial membrane and the permeability of the bacterial membrane and thus induced bacterial death. In addition, the ZnO/GO composites were found to be much less toxic to HeLa cells, compared to the equivalent concentration of ZnO NPs in the composites. The results indicate that the ZnO/GO composites are promising disinfection materials to be used in surface coatings on various substrates to effectively inhibit bacterial growth, propagation, and survival in medical devices.
具有良好抗菌活性和对其他物种较低毒性的新材料引起了众多研究兴趣。利用氧化锌 (ZnO) 和氧化石墨烯 (GO)，通过简便的一锅法反应制备了 ZnO/GO 复合材料，实现了优异的抗菌性能，而对其他物种没有损伤。在复合材料中，尺寸约为 4nm 的 ZnO 纳米颗粒 (NPs) 均匀锚定在 GO 片上。典型的细菌大肠杆菌和 HeLa 细胞分别用于评估 ZnO/GO 复合材料的抗菌活性和细胞毒性。GO 和 ZnO NPs 的协同作用导致了复合材料的优异抗菌活性。GO 有助于 ZnO NPs 的分散，减缓 ZnO 的溶解，充当溶解锌离子的储存场所，并使大肠杆菌与 ZnO NPs 和锌离子紧密接触。这种紧密接触增强了细菌细胞膜上局部锌浓度的点蚀和细胞膜的通透性，从而诱导细菌死亡。此外，与复合材料中 ZnO NPs 的等效浓度相比，ZnO/GO 复合材料对 HeLa 细胞的毒性要小得多。结果表明，ZnO/GO 复合材料有望成为各种基底表面涂层的消毒材料，以有效抑制医疗器械中细菌的生长、繁殖和存活。
