Laboratory of Nanoscale Characterization & Environmental Chemistry, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133-791, Republic of Korea.
Laboratory of Nanoscale Characterization & Environmental Chemistry, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133-791, Republic of Korea.
Colloids Surf B Biointerfaces. 2014 Apr 1;116:277-83. doi: 10.1016/j.colsurfb.2013.12.047. Epub 2014 Jan 18.
To improve our understanding on the impact of extrinsic properties of NPs on their bioaccumulation and toxicity, we have investigated the bioaccumulation of sub 100 nm sized P25 TiO₂ nanoparticles (NPs) by Daphnia magna (D. magna) in toxicity testing media. Based on our quantitative ICP-MS measurements as well as spectromicroscopic observations, we found that the bioaccumulation by D. magna were strongly influenced by the extrinsic properties of NPs as well as the biological uptake characteristics of D. magna. New sets of effective dosimetry parameters well correlated with the amount of NPs bioaccumulated within D. magna were also proposed. Based on these findings, we suggested that the extrinsic physicochemical properties of NPs (e.g., interfacial and colloidal properties of NPs) and biological characteristics for NPs (e.g., uptake cutoff sizes of testing organisms) should be considered and included when developing alternative dosimetry of NPs.
为了增进我们对纳米粒子（NPs）外在特性对其生物累积和毒性影响的理解，我们通过大型溞（Daphnia magna）在毒性测试介质中研究了亚 100nm 尺寸的 P25 TiO₂纳米粒子（NPs）的生物累积情况。基于我们的定量 ICP-MS 测量和光谱显微镜观察，我们发现 D. magna 的生物累积受到 NPs 的外在特性以及 D. magna 的生物摄取特征的强烈影响。还提出了一系列新的有效剂量学参数，这些参数与 D. magna 内累积的 NPs 量密切相关。基于这些发现，我们建议在开发 NPs 的替代剂量学方法时，应考虑 NPs 的外在物理化学特性（例如 NPs 的界面和胶体特性）和 NPs 的生物学特征（例如测试生物的摄取截止尺寸），并将其纳入其中。
