State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.
Planta Med. 2014 Feb;80(2-3):237-42. doi: 10.1055/s-0033-1360272. Epub 2014 Feb 4.
Minor phenolic compounds in licorice (Glycyrrhiza uralensis) have recently been proved for diverse bioactivities and favorable bioavailability, indicating that they may play an important role in the therapeutic effects or herb-drug interactions of licorice. However, so far, their abundance in licorice remains unknown. In this study, a reliable solid-phase extraction coupled with a high-performance liquid chromatography and diode array detection method was established to determine the minor phenolic compounds in licorice. The analytes were enriched by a three-step solid-phase extraction method, and then separated on a YMC ODS-A column by gradient elution. Five coumarins and flavonoids were identified by electrospray ionization tandem mass spectrometry, and then quantified using high-performance liquid chromatography and diode array detection. The amounts of glycycoumarin, dehydroglyasperin C, glycyrol, licoflavonol, and glycyrin in G. uralensis were 0.81 ± 0.28, 1.25 ± 0.59, 0.20 ± 0.08, 0.12 ± 0.04, and 0.17 ± 0.08 mg/g, respectively. Abundances of these compounds in other Glycyrrhiza species (G. glabra, G. inflata, and G. yunnanesis) were remarkably lower than G. uralensis.
甘草中的低酚类化合物具有多种生物活性和良好的生物利用度，这表明它们可能在甘草的治疗效果或草药-药物相互作用中发挥重要作用。然而，到目前为止，甘草中的低酚类化合物的含量仍不清楚。本研究建立了一种可靠的固相萃取-高效液相色谱-二极管阵列检测法，用于测定甘草中的低酚类化合物。采用三步固相萃取法对分析物进行富集，然后在 YMC ODS-A 柱上进行梯度洗脱分离。采用电喷雾串联质谱法鉴定了 5 种香豆素和黄酮类化合物，并采用高效液相色谱-二极管阵列检测法进行定量。甘草中的glycycoumarin、dehydroglyasperin C、glycyrol、licoflavonol 和glycyrin 的含量分别为 0.81±0.28、1.25±0.59、0.20±0.08、0.12±0.04 和 0.17±0.08mg/g。其他甘草属植物（G. glabra、G. inflata 和 G. yunnanesis）中这些化合物的含量明显低于甘草。
