Huo Yanghui, Li Kailin, Yang Suyu, Yi Bo, Chai Zhihua, Fan Lingxuan, Shu Liangyin, Gao Bowen, Li Huanting, Cai Wei
School of Pharmacy, Baotou Medical College, Baotou 014040, China.
School of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, China.
Molecules. 2024 Sep 13;29(18):4349. doi: 10.3390/molecules29184349.
The traditional Mongolian medicine Erdun-Uril is a conventional combination of 29 herbs commonly used for the treatment of cerebrovascular ailments. It has the effects of reducing inflammation, counteracting oxidative stress, and averting strokes caused by persistent cerebral hypoperfusion. Prior research on Erdun-Uril has predominantly concentrated on its pharmacodynamics and mechanism of action; however, there has been a lack of systematic and comprehensive investigation into its chemical constituents. Therefore, it is crucial to establish an efficient and rapid method for evaluating the chemical constituents of Erdun-Uril. In this study, Erdun-Uril was investigated using UHPLC-Q-Exactive Orbitrap MS combined with parallel reaction monitoring for the first time. Eventually, a total of 237 compounds, including 76 flavonoids, 68 phenolic compounds, 19 alkaloids, 7 amino acids, etc., were identified based on the chromatographic retention time, bibliography data, MS/MS information, neutral loss fragments (NLFs), and diagnostic fragment ions (DFIs). And of these, 225 were reported for the first time in this study. This new discovery of these complex components would provide a reliable theoretical basis for the development of pharmacodynamics and quality standards of the Mongolian medicine Erdun-Uril.
传统蒙药额尔敦-乌日勒是29味常用于治疗脑血管疾病的草药的传统组合。它具有抗炎、对抗氧化应激以及预防持续性脑灌注不足所致中风的作用。先前对额尔敦-乌日勒的研究主要集中在其药效学和作用机制方面；然而，对其化学成分缺乏系统全面的研究。因此，建立一种高效快速的额尔敦-乌日勒化学成分评价方法至关重要。在本研究中，首次采用超高效液相色谱-四极杆-静电场轨道阱质谱联用并行反应监测技术对额尔敦-乌日勒进行研究。最终，根据色谱保留时间、文献数据、二级质谱信息、中性丢失碎片（NLFs）和诊断性碎片离子（DFIs），共鉴定出237种化合物，包括76种黄酮类、68种酚类化合物、19种生物碱、7种氨基酸等。其中，225种是本研究首次报道。这些复杂成分的新发现将为蒙药额尔敦-乌日勒的药效学及质量标准的制定提供可靠的理论依据。
