Departament de Genètica i Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain,
Arch Microbiol. 2014 Mar;196(3):219-26. doi: 10.1007/s00203-014-0957-3. Epub 2014 Feb 5.
Drip irrigation systems using reclaimed water often present clogging events of biological origin. Microbial communities in biofilms from microirrigation systems of an experimental greenhouse in Almería, SE Spain, which used two different qualities of water (treated wastewater and reclaimed water), were analyzed by denaturing gradient gel electrophoresis and subsequent sequencing of amplified 16S rRNA gene bands. The most remarkable feature of all biofilms was that regardless of water origin, sequences belonging to Firmicutes were prevalent (53.5 % of total mean band intensity) and that almost all sequences recovered had some similarity (between 80.2 and 97 %) to thermophilic microorganisms. Mainly, sequences were closely related to potentially spore-forming organisms, suggesting that microbial communities able to grow at high temperatures were selected from the microbiota present in the incoming water. These pioneer results may contribute to improve management strategies to minimize the problems associated to biofouling in irrigation systems.
滴灌系统使用再生水常常会发生生物堵塞事件。通过变性梯度凝胶电泳和随后对扩增的 16S rRNA 基因条带进行测序，分析了来自西班牙东南部阿尔梅里亚一个实验温室微灌溉系统中生物膜的微生物群落，该系统使用了两种不同质量的水（处理后的废水和再生水）。所有生物膜最显著的特点是，无论水的来源如何，厚壁菌门的序列都占优势（占总平均带强度的 53.5%），并且几乎所有回收的序列都与嗜热微生物有一定的相似性（80.2%至 97%）。主要的是，序列与潜在的孢子形成生物密切相关，这表明能够在高温下生长的微生物群落是从进入水中的微生物群中选择出来的。这些初步结果可能有助于改进管理策略，以最大限度地减少灌溉系统中生物污垢相关问题。
