小麦V-H+-ATPase亚基基因显著影响拟南芥的耐盐性。
Wheat V-H+-ATPase subunit genes significantly affect salt tolerance in Arabidopsis thaliana.
作者信息
He Xiaoliang, Huang Xi, Shen Yinzhu, Huang Zhanjing
机构信息
School of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhang, Hebei, China.
College of Life Science of Hebei Normal University, Shijiazhuang, Hebei, China.
出版信息
PLoS One. 2014 Jan 30;9(1):e86982. doi: 10.1371/journal.pone.0086982. eCollection 2014.
Genes for V-H(+)-ATPase subunits were identified and cloned from the salt-tolerant wheat mutant RH8706-49. Sequences of these genes are highly conserved in plants. Overexpression of these genes in Arabidopsis thaliana improved its salt tolerance, and increased the activities of V-H(+)-ATPase and Na(+)/H(+) exchange, with the largest increase in plants carrying the c subunit of V-H(+)-ATPase. Results from quantitative RT-PCR analysis indicated that the mRNA level of each V-H(+)-ATPase subunit in the Arabidopsis increased under salt stress. Overall, our results suggest that each V-H(+)-ATPase subunit plays a key role in enhancing salt tolerance in plants.
从耐盐小麦突变体RH8706 - 49中鉴定并克隆了V - H(+)-ATP酶亚基的基因。这些基因的序列在植物中高度保守。在拟南芥中过表达这些基因提高了其耐盐性，并增加了V - H(+)-ATP酶和Na(+)/H(+)交换的活性，其中携带V - H(+)-ATP酶c亚基的植物增加幅度最大。定量RT - PCR分析结果表明，盐胁迫下拟南芥中每个V - H(+)-ATP酶亚基的mRNA水平均有所增加。总体而言，我们的结果表明，每个V - H(+)-ATP酶亚基在增强植物耐盐性方面都起着关键作用。
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