Narasimhan M L, Binzel M L, Perez-Prat E, Chen Z, Nelson D E, Singh N K, Bressan R A, Hasegawa P M
Center for Plant Environmental Stress Physiology, Department of Horticulture, Purdue University, West Lafayette, Indiana 47907.
Plant Physiol. 1991 Oct;97(2):562-8. doi: 10.1104/pp.97.2.562.
A cDNA clone encoding the 70-kilodalton subunit of the tobacco (Nicotiana tabacum var Wisconsin 38) tonoplast ATPase has been isolated. The 1.656 kilobase insert contains only open reading frame that represents more than 80% of the carrot cDNA coding region. The deduced amino acid sequence has greater than 95% sequence identity with the homologous carrot sequence. A transcript of approximately 2.7 kilobase was detected on Northern blots of tobacco poly(A)(+) selected or total RNA using labeled probe produced from this clone. The gene was expressed throughout the growth cycle in unadapted and 428 millimolar NaCl adapted cells. Transcription of the 70-kilodalton subunit gene or mRNA stability was induced by short-term NaCl treatment in NaCl adapted cells or by abscisic acid treatment in both adapted and unadapted cells. Southern analysis indicated the presence of up to four genes encoding the 70-kilodalton subunit.
已分离出一个编码烟草（烟草品种Wisconsin 38）液泡膜ATP酶70千道尔顿亚基的cDNA克隆。1.656千碱基的插入片段仅包含一个开放阅读框，该开放阅读框占胡萝卜cDNA编码区的80%以上。推导的氨基酸序列与同源胡萝卜序列的序列同一性大于95%。使用该克隆产生的标记探针，在烟草poly(A)(+)选择的或总RNA的Northern印迹上检测到约2.7千碱基的转录本。该基因在未适应和428毫摩尔NaCl适应的细胞的整个生长周期中均有表达。在NaCl适应的细胞中，短期NaCl处理或在适应和未适应的细胞中脱落酸处理均可诱导70千道尔顿亚基基因的转录或mRNA稳定性。Southern分析表明存在多达四个编码70千道尔顿亚基的基因。
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