College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030, China.
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, China.
Plant Cell Rep. 2018 Mar;37(3):443-452. doi: 10.1007/s00299-017-2241-x. Epub 2018 Jan 6.
Suppression of AtVHA-c5 expression results in changes in H and Na fluxes of roots, and increase sensitivity to salt in Arabidopsis. Vacuolar-type H-ATPase (V-ATPase), a multisubunit endomembrane proton pump, is essential in plant growth and response to environmental stresses. In the present study, the function of Arabidopsis V-ATPase subunit c5 (AtVHA-c5) gene in response to salt stress was investigated. Subcellular localization showed that AtVHA-c5 was mainly localized to endosomes and the vacuolar membrane in Arabidopsis. The analysis of quantitative real-time PCR showed that expression of AtVHA-c5 gene was induced by NaCl stress. Histochemical analysis revealed that AtVHA-c5 was expressed in the root epidermis of untreated Arabidopsis and in the whole root elongation zone after NaCl treatment. Phenotypic analysis showed that the atvha-c5 mutant is sensitive to high NaCl as compared to the wild type. The non-invasive micro-test technology measurement demonstrated that the net H and Na efflux in the root elongation zone of the atvha-c5 mutant was weaker than that of the wild type under NaCl treatment, suggesting that H and Na fluxes in atvha-c5 roots are impaired under NaCl stress. Moreover, compared to the wild type, the expression of AtSOS1 (salt overly sensitive 1) and AtAHA1 (plasma membrane H-ATPase 1) were down-regulated in atvha-c5 roots under NaCl stress. Overall, our results indicate that AtVHA-c5 plays a role in Arabidopsis root response to NaCl stress by influencing H and Na fluxes.
AtVHA-c5 表达抑制导致根中 H 和 Na 通量的变化，并增加拟南芥对盐的敏感性。液泡型 H+-ATP 酶（V-ATPase）是一种多亚基内膜质子泵，对于植物生长和对环境胁迫的反应至关重要。本研究探讨了拟南芥 V-ATPase 亚基 c5（AtVHA-c5）基因在盐胁迫响应中的功能。亚细胞定位表明，AtVHA-c5 主要定位于内体和拟南芥液泡膜。定量实时 PCR 分析表明，AtVHA-c5 基因的表达受 NaCl 胁迫诱导。组织化学分析显示，AtVHA-c5 在未处理的拟南芥根表皮和 NaCl 处理后的整个根伸长区表达。表型分析表明，与野生型相比，atvha-c5 突变体对高 NaCl 敏感。非侵入性微测试技术测量表明，在 NaCl 处理下，atvha-c5 突变体根伸长区的净 H 和 Na 外排比野生型弱，表明 NaCl 胁迫下 atvha-c5 根中的 H 和 Na 通量受损。此外，与野生型相比，在 NaCl 胁迫下，atvha-c5 根中的 AtSOS1（盐过度敏感 1）和 AtAHA1（质膜 H+-ATPase 1）的表达下调。总体而言，我们的结果表明，AtVHA-c5 通过影响 H 和 Na 通量在拟南芥根对 NaCl 胁迫的反应中发挥作用。
