实验性锌缺乏及补充对豚鼠某些免疫指标的影响。

Effect of experimental zinc deficiency and repletion on some immunological variables in guinea-pigs.

Verma P C, Gupta R P, Sadana J R, Gupta R K

Department of Veterinary Pathology, College of Veterinary Sciences, Haryana Agricultural University, Hisar, India.

Br J Nutr. 1988 Jan;59(1):149-54. doi: 10.1079/bjn19880018.

Abstract

Cellular and humoral immune responses were studied in guinea-pigs fed on zinc-deficient (ZnD), Zn-adequate (control) and Zn-replete diets containing 1.25, 50 and 100 mg Zn/kg diet respectively. 2. It was found that the ZnD guinea-pigs had significantly decreased ability to elicit delayed-type hypersensitivity (DTH) response against sheep erythrocytes as compared with controls on the 9th day of immunization. This was further substantiated by histological examination of DTH-positive skin sections. 3. A significant reduction in direct splenic plaque-forming-cell response and haemagglutinating-antibody titre was also observed in ZnD guinea-pigs. 4. Serum electrophoretic studies revealed a highly disordered protein profile with a significantly depressed value for gamma-globulin. 5. Zn repletion of the previously ZnD group resulted in marked, though incomplete, restoration of immunological responses.

摘要

分别以含1.25、50和100毫克锌/千克日粮的缺锌（ZnD）、锌充足（对照）和锌补充日粮喂养豚鼠，研究其细胞免疫和体液免疫反应。2. 结果发现，与对照组相比，在免疫第9天时，ZnD豚鼠对绵羊红细胞引发迟发型超敏反应（DTH）的能力显著降低。DTH阳性皮肤切片的组织学检查进一步证实了这一点。3. 在ZnD豚鼠中还观察到直接脾斑形成细胞反应和血凝抗体滴度显著降低。4. 血清电泳研究显示蛋白质谱高度紊乱，γ球蛋白值显著降低。5. 对先前的ZnD组补充锌后，免疫反应显著但未完全恢复。