Universidade Federal de Uberlândia, Instituto de Ciências Biomédicas - Área de Fisiologia e Farmacologia Av, Pará, 1720 Campus Umuruama, CEP: 38400-902 Uberlândia-MG, Brazil.
Diabetol Metab Syndr. 2013 Oct 24;5(1):64. doi: 10.1186/1758-5996-5-64.
Oral health complications in diabetes and hypertension include decreased salivary secretion. The sodium-glucose cotransporter 1 (SGLT1) protein, which transports 1 glucose/2 Na+/264 H2O molecules, is described in salivary glands. We hypothesized that changes in SGLT1 expression in the luminal membrane of ductal cell may be related to an altered salivary flow.
By immunohistochemistry, we investigated SGLT1 expression in ductal cells of parotid and submandibular glands from Wistar Kyoto rats (WKY), diabetic WKY (WKY-D), spontaneously hypertensive rats (SHR) and diabetic SHR (SHR-D), as well as in parotid glands from WKY subjected to sympathetic stimulation, with or without previous propranolol blockade. Diabetes and hypertension decreased the salivary secretion and increased SGLT1 expression in the luminal membrane of ductal cells, and their association exacerbated the regulations observed. After 30 min of sympathetic stimulation, SGLT1 increased in the luminal membrane of ductal cells, and that was blocked by previous injection of propranolol.
SGLT1 expression increases in the luminal membrane of salivary gland ductal cells and the salivary flow decreases in diabetic and hypertensive rats, which may be related to sympathetic activity. This study highlights the water transporter role of SGLT1 in salivary glands, which, by increasing ductal water reabsorption, may explain the hyposalivation of diabetic and hypertensive subjects.
糖尿病和高血压的口腔健康并发症包括唾液分泌减少。钠-葡萄糖共转运蛋白 1（SGLT1）蛋白在唾液腺中被描述为转运 1 个葡萄糖/2 个 Na+/264 个 H2O 分子。我们假设，导管细胞腔膜中 SGLT1 表达的变化可能与唾液流量的改变有关。
通过免疫组织化学，我们研究了 Wistar Kyoto 大鼠（WKY）、糖尿病 Wistar Kyoto 大鼠（WKY-D）、自发性高血压大鼠（SHR）和糖尿病自发性高血压大鼠（SHR-D）腮腺和颌下腺导管细胞中 SGLT1 的表达，以及交感神经刺激后的 WKY 腮腺中 SGLT1 的表达，同时或不预先注射普萘洛尔阻断。糖尿病和高血压降低了唾液分泌量，并增加了导管细胞腔膜中的 SGLT1 表达，其相关性加剧了观察到的调节。在交感神经刺激 30 分钟后，导管细胞腔膜中的 SGLT1 增加，而这一增加被先前注射普萘洛尔所阻断。
糖尿病和高血压大鼠唾液腺导管细胞腔膜中的 SGLT1 表达增加，唾液流量减少，这可能与交感神经活动有关。本研究强调了 SGLT1 在唾液腺中的水转运蛋白作用，通过增加导管水重吸收，可能解释了糖尿病和高血压患者的唾液减少。
