Li Xin, Shi Zhongping, Li Zhigang
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Bioprocess Biosyst Eng. 2014 Aug;37(8):1609-16. doi: 10.1007/s00449-014-1133-5. Epub 2014 Feb 7.
In this study, we attempted to increase butanol/acetone ratio and total solvent productivity in ABE fermentations with corn- and cassava-based media, by consecutively feeding a small amount of butyrate/acetate during solventogenic phase to weaken the metabolic strengths in butyrate/acetate closed-loops. Consecutively feeding a small amount of butyrate (a total of 3.0 g/L-broth) is most effective in improving performance of corn-based ABE fermentations, as it simultaneously increased average butanol/acetone ratio by 23 % (1.92-2.36) and total solvent productivity by 16 % (0.355-0.410 g/L/h) as compared with those of control. However, the butyrate feeding strategy could not improve butanol/acetone ratio and total solvent productivity in cassava-based ABE fermentations, where the metabolic strength of butyrate closed-loop had already been very low.
在本研究中，我们试图通过在溶剂生成阶段连续少量添加丁酸盐/乙酸盐，以削弱丁酸盐/乙酸盐闭环中的代谢强度，从而提高以玉米和木薯为原料的培养基在ABE发酵中的丁醇/丙酮比例以及总溶剂生产率。连续添加少量丁酸盐（总计3.0 g/L发酵液）对改善以玉米为原料的ABE发酵性能最为有效，与对照相比，它同时使平均丁醇/丙酮比例提高了23%（从1.92提高到2.36），总溶剂生产率提高了16%（从0.355 g/L/h提高到0.410 g/L/h）。然而，丁酸盐添加策略无法提高以木薯为原料的ABE发酵中的丁醇/丙酮比例和总溶剂生产率，因为在这种发酵中丁酸盐闭环的代谢强度已经非常低。
