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Topical Treatment of Dermatophytic Lesion on Mice (Mus musculus) Model.小鼠（Mus musculus）模型中皮肤真菌病损的局部治疗。
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Medico-botanical study of Yercaud hills in the eastern Ghats of Tamil Nadu, India.印度泰米尔纳德邦东高止山脉耶尔考德山的药用植物学研究。
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Evaluation of wound healing potential of topical formulation of leaf juice of tridax procumbens L. In mice.三叶鬼针草叶汁局部制剂对小鼠伤口愈合潜力的评估。
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Dynamics of seed-borne rice endophytes on early plant growth stages.种子携带的水稻内生菌对早期植物生长阶段的影响。
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Antiplasmodial activity of extracts of Tridax procumbens and Phyllanthus amarus in in vitro Plasmodium falciparum culture systems.黄花稔和苦味叶下珠提取物在体外恶性疟原虫培养系统中的抗疟活性。
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Diversity and antimicrobial activities of the fungal endophyte community associated with the traditional Brazilian medicinal plant Solanum cernuum Vell. (Solanaceae).与传统的巴西药用植物 Solanum cernuum Vell.（茄科）相关的真菌内生菌群落的多样性和抗菌活性。
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与传统药用植物三叶鬼针草相关的内生细菌的鉴定

Identification of bacterial endophytes associated with traditional medicinal plant Tridax procumbens Linn.

作者信息

Preveena Jagadesan, Bhore Subhash J

机构信息

Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong, Kedah, Malaysia.

出版信息

Anc Sci Life. 2013 Jan;32(3):173-7. doi: 10.4103/0257-7941.123002.

DOI:10.4103/0257-7941.123002
PMID:24501447
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3902539/
Abstract

BACKGROUND

In traditional medicine, Tridax procumbens Linn. is used in the treatment of injuries and wounds. The bacterial endophytes (BEs) of medicinal plants could produce medicinally important metabolites found in their hosts; and hence, the involvement of BEs in conferring wound healing properties to T. Procumbens cannot be ruled out. But, we do not know which types of BEs are associated with T. Procumbens.

OBJECTIVE

The objective of this study was to investigate the fast growing and cultivable BEs associated with T. procumbens.

MATERIALS AND METHODS

Leaves and stems of healthy T. Procumbens plants were collected and cultivable BEs were isolated from surface-sterilized leaf and stem tissue samples using Luria-Bertani (LB) agar (medium) at standard conditions. A polymerase chain reaction was employed to amplify 16S rRNA coding gene fragments from the isolates. Cultivable endophytic bacterial isolates (EBIs) were identified using 16S rRNA gene nucleotide sequence similarity based method of bacterial identification.

RESULTS

Altogether, 50 culturable EBIs were isolated. 16S rRNA gene nucleotide sequences analysis using the Basic Local Alignment Search Tool (BLAST) revealed identities of the EBIs. Analysis reveals that cultivable Bacillus spp., Cronobacter sakazakii, Enterobacter spp., Lysinibacillus sphaericus, Pantoea spp., Pseudomonas spp. and Terribacillus saccharophilus are associated with T. Procumbens.

CONCLUSION

Based on the results, we conclude that 24 different types of culturable BEs are associated with traditionally used medicinal plant, T. Procumbens, and require further study.

摘要

背景

在传统医学中，平卧银胶菊用于治疗损伤和伤口。药用植物的细菌内生菌可产生其宿主中发现的具有重要药用价值的代谢产物；因此，不能排除内生菌在赋予平卧银胶菊伤口愈合特性方面的作用。但是，我们尚不清楚哪些类型的内生菌与平卧银胶菊相关。

目的

本研究旨在调查与平卧银胶菊相关的快速生长且可培养的内生菌。

材料与方法

采集健康平卧银胶菊植株的叶片和茎，在标准条件下，使用Luria-Bertani（LB）琼脂（培养基）从表面消毒的叶和茎组织样本中分离可培养的内生菌。采用聚合酶链反应从分离株中扩增16S rRNA编码基因片段。使用基于16S rRNA基因核苷酸序列相似性的细菌鉴定方法对可培养的内生细菌分离株（EBI）进行鉴定。

结果

共分离出50株可培养的EBI。使用基本局部比对搜索工具（BLAST）对16S rRNA基因核苷酸序列进行分析，揭示了EBI的身份。分析表明，可培养的芽孢杆菌属、阪崎肠杆菌、肠杆菌属、球形赖氨酸芽孢杆菌、泛菌属、假单胞菌属和嗜糖栖芽孢杆菌与平卧银胶菊相关。

结论

基于这些结果，我们得出结论，24种不同类型的可培养内生菌与传统药用植物平卧银胶菊相关，需要进一步研究。