Bibi Fehmida
Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
Saudi J Biol Sci. 2017 Sep;24(6):1288-1293. doi: 10.1016/j.sjbs.2015.09.021. Epub 2015 Oct 1.
The antimicrobial activity of plant extract of , a medicinal plant has been studied already. However, knowledge about bacterial diversity associated with different parts of host plant antagonistic to different human pathogenic bacteria is limited. In this study, bacteria were isolated from root, leaf and fruit of plant. Among 188 bacterial isolates isolated from different parts of the plant only 24 were found to be active against different pathogenic bacteria i.e. , Methicillin-resistant (MRSA), , and . These active bacterial isolates were identified on the basis of 16S rRNA gene analysis. Total population of bacteria isolated from plant was high in root, following leaf and fruit. Antagonistic bacteria were also more abundant in root as compared to leaf and fruit. Two isolates (EA5 and EA18) exhibited antagonistic activity against most of the targeted pathogenic bacteria mentioned above. Some isolates showed strong inhibition for one targeted pathogenic bacterium while weak or no inhibition for others. Most of the antagonistic isolates were active against MRSA, following , , and . Taken together, our results show that medicinal plants are good source of antagonistic bacteria having inhibitory effect against clinical bacterial pathogens.
一种药用植物的植物提取物的抗菌活性已经得到研究。然而，关于与宿主植物不同部位相关的、对不同人类病原菌具有拮抗作用的细菌多样性的知识有限。在本研究中，从该植物的根、叶和果实中分离细菌。从植物不同部位分离出的188株细菌分离物中，仅发现24株对不同病原菌即耐甲氧西林金黄色葡萄球菌（MRSA）、大肠杆菌、肺炎克雷伯菌和铜绿假单胞菌有活性。这些有活性的细菌分离物基于16S rRNA基因分析进行鉴定。从该植物分离出的细菌总数在根中最高，其次是叶和果实。与叶和果实相比，根中的拮抗细菌也更为丰富。两个分离物（EA5和EA18）对上述大多数目标病原菌表现出拮抗活性。一些分离物对一种目标病原菌表现出强烈抑制作用，而对其他病原菌则表现出微弱抑制作用或无抑制作用。大多数拮抗分离物对MRSA有活性，其次是大肠杆菌、肺炎克雷伯菌和铜绿假单胞菌。综上所述，我们的结果表明，药用植物是具有抑制临床细菌病原体作用的拮抗细菌的良好来源。
