Kleinert H, Benecke B J
Lehrstuhl für Biochemie, Ruhr-Universität, FRG.
Nucleic Acids Res. 1988 Feb 25;16(4):1319-31. doi: 10.1093/nar/16.4.1319.
We have analyzed the transcription of a recently isolated human 7S K RNA gene in vitro and in vivo. In contrast to hitherto characterized class III genes (genes transcribed by RNA polymerase III), the coding sequence of this gene is not required for faithful and efficient transcription by RNA polymerase III. In fact, a procaryotic vector DNA sequence was efficiently transcribed by RNA polymerase III under the control of the 7S K RNA gene upstream sequence in vitro and in vivo. S1-nuclease protection analyses confirmed that the 7S K 5'flanking sequence was sufficient for accurate transcription initiation. These data demonstrate that 7S K DNA represents a novel class III gene, the promoter elements of which are located outside the coding sequence.
我们已经分别在体外和体内对最近分离出的人类7S K RNA基因的转录情况进行了分析。与迄今已鉴定的III类基因（由RNA聚合酶III转录的基因）不同，该基因的编码序列对于RNA聚合酶III进行准确且高效的转录并非必需。事实上，在体外和体内，原核载体DNA序列在7S K RNA基因上游序列的控制下，都能被RNA聚合酶III高效转录。S1核酸酶保护分析证实，7S K 5'侧翼序列足以实现精确的转录起始。这些数据表明，7S K DNA代表了一种新型的III类基因，其启动子元件位于编码序列之外。
