Department of Biology, Facultad de Química, UNAM, Ciudad Universitaria, México D.F. 04510, Mexico.
Food Funct. 2014 Mar;5(3):480-90. doi: 10.1039/c3fo60501a.
The aim of this study was to evaluate whether the antioxidant C-phycocyanin (C-PC, 5-30 mg kg(-1) i.p.) was able to prevent cisplatin (CP, 18 mg kg(-1) i.p.) induced nephrotoxicity by reducing oxidative stress in CD-1 mice. Nephrotoxicity was assessed by measuring blood urea nitrogen, plasma glutathione peroxidase, plasma creatinine, the renal activity of N-acetyl-β-d-glucosaminidase, apoptosis and histopathological changes. Oxidative stress was evaluated by measuring the content of glutathione, malondialdehyde, 4-hydroxynonenal and oxidized proteins in renal tissue. C-PC prevented CP-induced renal damage and oxidative stress in a dose-dependent manner. Moreover, C-PC prevented the decrease in the renal activity of the antioxidant enzymes glutathione peroxidase, glutathione reductase, glutathione-S-transferase and catalase induced by cisplatin. In vitro assays showed that C-PC was an effective scavenger of the following reactive species: hypochlorous acid, peroxynitrite anions, peroxyl radicals, diphenyl-1-picrylhydrazyl, hydroxyl radicals, superoxide anions, singlet oxygen and hydrogen peroxide. It is concluded that the protective effect of the nutraceutical C-PC against CP-induced nephrotoxicity was associated with the attenuation of oxidative stress and the preservation of the activity of antioxidant enzymes.
本研究旨在评估抗氧化剂 C-藻蓝蛋白（C-PC，5-30mg/kg 腹腔注射）是否能够通过减少 CD-1 小鼠的氧化应激来预防顺铂（CP，18mg/kg 腹腔注射）诱导的肾毒性。通过测量血尿素氮、血浆谷胱甘肽过氧化物酶、血浆肌酐、肾 N-乙酰-β-d-氨基葡萄糖苷酶活性、细胞凋亡和组织病理学变化来评估肾毒性。通过测量肾组织中谷胱甘肽、丙二醛、4-羟基壬烯醛和氧化蛋白的含量来评估氧化应激。C-PC 以剂量依赖的方式预防 CP 诱导的肾损伤和氧化应激。此外，C-PC 预防了 CP 诱导的抗氧化酶谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽-S-转移酶和过氧化氢酶活性的降低。体外试验表明，C-PC 是以下活性物质的有效清除剂：次氯酸、过氧亚硝酸阴离子、过氧自由基、二苯-1-苦肼基、羟基自由基、超氧阴离子、单线态氧和过氧化氢。因此，营养保健品 C-PC 对 CP 诱导的肾毒性的保护作用与减轻氧化应激和维持抗氧化酶活性有关。
