Akura K, Hatakenaka M, Kawai K, Takenaka M, Kato K
Section of Cytology and Pathology, Osaka Teishin Hospital, Japan.
Acta Cytol. 1988 Mar-Apr;32(2):247-51.
The application of immunocytochemistry in urinary cytology for the identification of a human polyomavirus infection is described. The Papanicolaoustained slides of voided urine specimens of a 26-year-old man undergoing steroid therapy showed many inclusion-bearing epithelial cells. After subsequent destaining of the same slides, the presence of human papovavirus antigen in the nuclei of infected exfoliated cells was demonstrated by immunocytochemical staining with simian virus 40 antiserum and peroxidase. Papovaviruses were also detected by electron microscopic study of the smears, confirming the diagnosis of a human polyomavirus infection. The use of immunoperoxidase studies proved to be advantageous for the rapid cytodiagnosis of human polyomavirus infection in the urinary specimens in this case; such studies may be of particular value in equivocal cases to prove or disprove the viral nature of morphologic changes observed in routine preparations.
本文描述了免疫细胞化学在尿细胞学中用于鉴定人多瘤病毒感染的应用。一名接受类固醇治疗的26岁男性的排空尿液标本经巴氏染色的玻片显示有许多含包涵体的上皮细胞。在对同一张玻片进行后续脱色后，用猴病毒40抗血清和过氧化物酶进行免疫细胞化学染色，证明感染的脱落细胞核中存在人乳头多瘤空泡病毒抗原。通过对涂片的电子显微镜研究也检测到了乳头多瘤空泡病毒，证实了人多瘤病毒感染的诊断。在该病例中，免疫过氧化物酶研究被证明有利于对尿液标本中的人多瘤病毒感染进行快速细胞诊断；此类研究在疑难病例中对于证实或否定常规制片中观察到的形态学改变的病毒性质可能具有特殊价值。
