Dupuy Célie, Galland Claire, Devaux Alain, Bony Sylvie, Loizeau Véronique, Danion Morgane, Pichereau Vianney, Fournier Michel, Laroche Jean
UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l'Environnement Marin LEMAR, Institut Universitaire Européen de la Mer, Université Européenne de Bretagne, Université de Bretagne occidentale, Plouzané, 29280, France,
Environ Sci Pollut Res Int. 2014 Dec;21(24):13789-803. doi: 10.1007/s11356-014-2563-y. Epub 2014 Feb 7.
A multibiomarker approach was developed to evaluate the juvenile European flounder responses to a complex mixture of 9 polycyclic aromatic hydrocarbons (PAHs) and 12 polychlorinated biphenyls (PCBs). Exposure was performed through contaminated food pellets displaying: (1) PAH and PCB levels similar to those detected in the heavily polluted Seine estuary, respectively in sediments and in flatfish and (2) ten times these concentrations. Several biomarkers of the immune system (e.g., lysozyme concentration and gene expression of complement component C3 and TNF-receptor), DNA damage (e.g., Comet assay), energetic metabolism (e.g., activity of cytochrome C oxidase), detoxification process (e.g., cytochrome P450 1A1 expression level: CYP1A1; betaine homocysteine methyl transferase expression level: BHMT) were investigated after 14 and 29 days of contamination, followed by a 14-days recovery period. After 29 days of contamination, the detoxification activity (CYP1A1 expression level) was positively correlated with DNA damages; the increase of the BHMT expression level could also be related to the detoxification process. Furthermore, after the recovery period, some biomarkers were still upregulated (i.e., CYP1A1 and BHMT expression levels). The immune system was significantly modulated by the chemical stress at the two concentration levels, and the lysozyme appeared to be the most sensitive marker of the mixture impact.
开发了一种多生物标志物方法来评估欧洲幼比目鱼对9种多环芳烃（PAHs）和12种多氯联苯（PCBs）复杂混合物的反应。通过受污染的食物颗粒进行暴露，这些颗粒显示出：（1）PAH和PCB水平分别与在严重污染的塞纳河口沉积物和平鱼中检测到的水平相似，以及（2）这些浓度的十倍。在污染14天和29天后，接着是14天的恢复期，研究了免疫系统的几种生物标志物（例如，溶菌酶浓度以及补体成分C3和TNF受体的基因表达）、DNA损伤（例如，彗星试验）、能量代谢（例如，细胞色素C氧化酶的活性）、解毒过程（例如，细胞色素P450 1A1表达水平：CYP1A1；甜菜碱同型半胱氨酸甲基转移酶表达水平：BHMT）。污染29天后，解毒活性（CYP1A1表达水平）与DNA损伤呈正相关；BHMT表达水平的增加也可能与解毒过程有关。此外，在恢复期后，一些生物标志物仍然上调（即CYP1A1和BHMT表达水平）。在两个浓度水平下，免疫系统均受到化学应激的显著调节，溶菌酶似乎是混合物影响的最敏感标志物。
