Khamaiseh Emran Issa Said, Hamid Aidil Abd, Yusoff Wan Mohtar Wan, Kalil Mohd Sahaid
Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia.
School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia.
Pak J Biol Sci. 2013 Oct 15;16(20):1145-51. doi: 10.3923/pjbs.2013.1145.1151.
Date fruit juice contains high concentration of simple sugars ranging from 65 to 75% (w/w) in dry form. In this study, the potential of date fruit juice as biobutanol fermentation medium by C. acetobutylicum was investigated. The fermentation process was carried out at initial pH of 5, 6 and 7, incubation temperature of 30, 35 and 40 degrees C for 72 hours. The date fruit concentrations tested were 10, 20, 30 and 40 g L(-1). Medium containing 30 g L(-1) of date fruit at 35 degrees C incubation temperature with initial medium pH 7.0 gave the highest concentration of solvents of 3.1, 0.1 and 1.1 g L(-1) butanol, ethanol and acetone respectively. The yield and productivity of biobutanol were 0.32 g g(-1) and 0.044 g L(-1)/h respectively, while for total ABE were 0.45 g g(-1) and 0.06 g L(-1) h, respectively.
椰枣果汁含有高浓度的单糖，以干重计，其含量在65%至75%（w/w）之间。在本研究中，研究了椰枣果汁作为丙酮丁醇梭菌生物丁醇发酵培养基的潜力。发酵过程在初始pH值为5、6和7、培养温度为30、35和40摄氏度的条件下进行72小时。所测试的椰枣果汁浓度为10、20、30和40 g L(-1)。在35摄氏度培养温度、初始培养基pH值为7.0的条件下，含有30 g L(-1)椰枣果汁的培养基产生的溶剂浓度最高，丁醇、乙醇和丙酮分别为3.1、0.1和1.1 g L(-1)。生物丁醇的产量和生产率分别为0.32 g g(-1)和0.044 g L(-1)/h，而总ABE的产量和生产率分别为0.45 g g(-1)和0.06 g L(-1) h。
