Khamaiseh Emran I, Abdul Hamid Aidil, Abdeshahian Peyman, Wan Yusoff Wan Mohtar, Kalil Mohd Sahaid
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
ScientificWorldJournal. 2014 Jan 6;2014:395754. doi: 10.1155/2014/395754. eCollection 2014.
The production of biobutanol was studied by the cultivation of Clostridium acetobutylicum NCIMB 13557 in P2 medium including date fruit as the sole substrate. The effect of P2 medium and the effect of different concentrations of date fruit ranging from 10 to 100 g/L on biobutanol production were investigated. Anaerobic batch culture was carried out at 35 °C incubation temperature and pH 7.0 ± 0.2 for 72 h. Experimental results showed that the lowest yield of biobutanol and acetone-butanol-ethanol (ABE) was 0.32 and 0.35 gram per gram of carbohydrate consumed (g/g), respectively, when an initial date fruit concentration of 10 g/L was utilized. At this fruit date concentration a biobutanol production value of 1.56 g/L was obtained. On the other hand, the maximum yield of biobutanol (0.48 g/g) and ABE (0.63 g/g) was produced at 50 g/L date fruit concentration with a biobutanol production value as high as 11 g/L. However, when a higher initial date fruit concentration was used, biobutanol and ABE production decreased to reach the yield of 0.22 g/g and 0.35 g/g, respectively, where 100 g/L date fruit was used. Similar results also revealed that 10.03 g/L biobutanol was produced using 100 g/L date fruit.
通过在以枣果为唯一底物的P2培养基中培养丙酮丁醇梭菌NCIMB 13557来研究生物丁醇的生产。研究了P2培养基以及10至100 g/L不同浓度枣果对生物丁醇生产的影响。在35°C培养温度和pH 7.0±0.2条件下进行厌氧分批培养72小时。实验结果表明，当初始枣果浓度为10 g/L时，每克消耗的碳水化合物产生的生物丁醇和丙酮 - 丁醇 - 乙醇（ABE）的最低产量分别为0.32克和0.35克（g/g）。在此枣果浓度下，生物丁醇产量为1.56 g/L。另一方面，在枣果浓度为50 g/L时，生物丁醇（0.48 g/g）和ABE（0.63 g/g）的产量最高，生物丁醇产量高达11 g/L。然而，当使用更高的初始枣果浓度时，生物丁醇和ABE产量下降，在使用100 g/L枣果时，产量分别降至0.22 g/g和0.35 g/g。类似结果还表明，使用100 g/L枣果时可产生10.03 g/L生物丁醇。
