Yewdell J W, Yellen A, Bächi T
Wistar Institute for Anatomy and Biology, Philadelphia, Pennsylvania 19104.
Cell. 1988 Mar 25;52(6):843-52. doi: 10.1016/0092-8674(88)90426-6.
We used monoclonal antibodies that recognize monomeric and/or trimeric forms of the influenza virus hemagglutinin (HA) to study biosynthesis of this integral membrane protein in influenza virus-infected cells. We find the following: First, the globular head of the HA folds into its mature conformation in the endoplasmic reticulum prior to the assembly of HA monomers into trimers. Second, trimerization begins within 1 to 2 min following synthesis, with a half-time of approximately 5 min. Third, trimerization occurs only after the HA has been transported from the endoplasmic reticulum. Fourth, newly formed trimers are sensitive to acid-induced conformational alterations associated with viral fusion activity.
我们使用了可识别流感病毒血凝素（HA）单体和/或三聚体形式的单克隆抗体，来研究这种整合膜蛋白在流感病毒感染细胞中的生物合成。我们发现以下几点：第一，HA的球状头部在内质网中在HA单体组装成三聚体之前就折叠成其成熟构象。第二，三聚化在合成后1至2分钟内开始，半衰期约为5分钟。第三，三聚化仅在HA从内质网转运之后发生。第四，新形成的三聚体对与病毒融合活性相关的酸诱导构象改变敏感。
