混合流感血凝素三聚体的翻译后寡聚化及协同酸激活作用
Posttranslational oligomerization and cooperative acid activation of mixed influenza hemagglutinin trimers.
作者信息
Boulay F, Doms R W, Webster R G, Helenius A
机构信息
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510.
出版信息
J Cell Biol. 1988 Mar;106(3):629-39. doi: 10.1083/jcb.106.3.629.
Abstract
The influenza virus hemagglutinin (HA) is a well-characterized integral membrane glycoprotein composed of three identical subunits. We have analyzed the formation of mixed trimers in cells expressing two different HA gene products. The results show efficient and essentially random assembly of functional hybrid trimers provided that the HAs are from the same HA subtype. Trimerization is thus a posttranslational event, and subunits are recruited randomly from a common pool of monomers in the endoplasmic reticulum. Mixed trimers were not observed between HAs derived from different subtypes, indicating that the trimerization event is sequence specific. Mixed trimers containing mutant subunits were, moreover, used to establish that the acid-induced conformational change involved in the membrane fusion activity of HA is a highly cooperative event.
摘要
流感病毒血凝素（HA）是一种特征明确的整合膜糖蛋白，由三个相同的亚基组成。我们分析了表达两种不同HA基因产物的细胞中混合三聚体的形成情况。结果表明，只要HA来自同一HA亚型，功能性杂合三聚体就能高效且基本随机地组装。因此，三聚化是一个翻译后事件，亚基从内质网中共同的单体池中随机募集。在源自不同亚型的HA之间未观察到混合三聚体，这表明三聚化事件具有序列特异性。此外，含有突变亚基的混合三聚体被用于证实，HA膜融合活性中涉及的酸诱导构象变化是一个高度协同的事件。
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本文引用的文献
1
Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution.流感病毒血凝素膜糖蛋白在3埃分辨率下的结构
Nature. 1981 Jan 29;289(5796):366-73. doi: 10.1038/289366a0.
2
Influenza viruses cause hemolysis and fusion of cells.流感病毒会引起细胞溶血和融合。
Virology. 1981 Apr 15;110(1):243-7. doi: 10.1016/0042-6822(81)90030-1.
3
Changes in the conformation of influenza virus hemagglutinin at the pH optimum of virus-mediated membrane fusion.在病毒介导的膜融合的最适pH值下流感病毒血凝素构象的变化
Proc Natl Acad Sci U S A. 1982 Feb;79(4):968-72. doi: 10.1073/pnas.79.4.968.
4
Interaction of influenza virus hemagglutinin with target membrane lipids is a key step in virus-induced hemolysis and fusion at pH 5.2.流感病毒血凝素与靶膜脂质的相互作用是病毒诱导的溶血以及在pH 5.2时发生融合的关键步骤。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4133-7. doi: 10.1073/pnas.78.7.4133.
5
Reduced temperature prevents transfer of a membrane glycoprotein to the cell surface but does not prevent terminal glycosylation.降低温度会阻止膜糖蛋白转移至细胞表面，但不会阻止其末端糖基化。
Cell. 1983 Aug;34(1):233-43. doi: 10.1016/0092-8674(83)90154-x.
6
Haemagglutinin of influenza virus expressed from a cloned gene promotes membrane fusion.从克隆基因表达的流感病毒血凝素可促进膜融合。
Nature. 1982 Dec 16;300(5893):658-9. doi: 10.1038/300658a0.
7
Intracellular transport of influenza virus hemagglutinin to the apical surface of Madin-Darby canine kidney cells.流感病毒血凝素在细胞内运输至犬肾传代细胞（Madin-Darby canine kidney cells）顶端表面的过程。
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Density of newly synthesized plasma membrane proteins in intracellular membranes II. Biochemical studies.细胞内膜中新合成的质膜蛋白的密度II. 生化研究
J Cell Biol. 1984 Jun;98(6):2142-7. doi: 10.1083/jcb.98.6.2142.
9
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10
Membrane fusion proteins of enveloped animal viruses.包膜动物病毒的膜融合蛋白。
Q Rev Biophys. 1983 May;16(2):151-95. doi: 10.1017/s0033583500005072.
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