Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, 59 Cangwu Road, Lianyungang 222005, China; Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
Carbohydr Polym. 2014 Feb 15;102:649-52. doi: 10.1016/j.carbpol.2013.11.010. Epub 2013 Nov 15.
A strain producing chitin deacetylase (CDA) was isolated and identified as Rhodococcus erythropolis by morphological characteristics and 16S rDNA analysis, named as R. erythropolis HG05. By Plackett-Burman and central composite design, CDA production from R. erythropolis HG05 was increased from 58.00 U/mL to 238.89 U/mL. With the crude enzyme from R. erythropolis HG05, the hydrolysate components from colloid chitin were chito-oligosaccharides with polymerization number larger than hexaose.
一株产几丁质脱乙酰酶（CDA）的菌株，通过形态特征和 16S rDNA 分析，被鉴定为红球菌属，命名为 R. erythropolis HG05。通过 Plackett-Burman 和中心复合设计，R. erythropolis HG05 的 CDA 产量从 58.00 U/mL 提高到 238.89 U/mL。利用 R. erythropolis HG05 的粗酶，胶体几丁质水解产物的组分为聚合度大于六糖的壳寡糖。
