从红球菌 HG05 中生产壳聚糖脱乙酰酶的统计优化。
Statistical optimization for production of chitin deacetylase from Rhodococcus erythropolis HG05.
机构信息
Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, 59 Cangwu Road, Lianyungang 222005, China; Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
出版信息
Carbohydr Polym. 2014 Feb 15;102:649-52. doi: 10.1016/j.carbpol.2013.11.010. Epub 2013 Nov 15.
A strain producing chitin deacetylase (CDA) was isolated and identified as Rhodococcus erythropolis by morphological characteristics and 16S rDNA analysis, named as R. erythropolis HG05. By Plackett-Burman and central composite design, CDA production from R. erythropolis HG05 was increased from 58.00 U/mL to 238.89 U/mL. With the crude enzyme from R. erythropolis HG05, the hydrolysate components from colloid chitin were chito-oligosaccharides with polymerization number larger than hexaose.
一株产几丁质脱乙酰酶（CDA）的菌株，通过形态特征和 16S rDNA 分析，被鉴定为红球菌属，命名为 R. erythropolis HG05。通过 Plackett-Burman 和中心复合设计，R. erythropolis HG05 的 CDA 产量从 58.00 U/mL 提高到 238.89 U/mL。利用 R. erythropolis HG05 的粗酶，胶体几丁质水解产物的组分为聚合度大于六糖的壳寡糖。
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