人脐带间充质干细胞对放射性肺炎的实验性治疗

Experimental treatment of radiation pneumonitis with human umbilical cord mesenchymal stem cells.

作者信息

Wang Rui, Zhu Chang-zheng, Qiao Ping, Liu Jian, Zhao Qiang, Wang Kui-jie, Zhao Ting-bao

机构信息

Department of Spinal Cord Repairing, Jinan Military General Hospital, Jinan 250031, China.

Department of Neurology, People's Hospital of Zhangqiu, Zhangqiu 250200, China.

出版信息

Asian Pac J Trop Med. 2014 Apr;7(4):262-6. doi: 10.1016/S1995-7645(14)60034-1.

DOI:10.1016/S1995-7645(14)60034-1

PMID:24507672

Abstract

OBJECTIVE

To evaluate of the curative effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) on rat acute radiation pneumonitis.

METHODS

Fourty rats were randomly divided into control group, radiation group, stem cell prevention group, stem cell treatment group and prednisone treatment group. All rats except those in the control group were radiated with X ray to establish the acute radiation pneumonitis damage model. The hUC-MSCs cultured in vitro was administrated to the rats of the prevention group via tail vein (1×10(6) cells/kg BW) 24 h before the radiation, while the same administration was performed in the rats of the treatment group 24 h after the radiation. After 24 h post the radiation, the rats in the radiation group were given 0.4 mL physiological saline, and those in the prednisone group were given 1 mg/kg prednisone. All rats were observed and executed 72 h after the radiation to detect lung histological changes.

RESULTS

After the administration of hUC-MSCs, the survival status of the rats in the prevention group and treatment group was obviously better than that in the control group. As shown by the histological staining, the morphology, proliferation activity and bronchial state of lung tissues were better in the prevention group and treatment group than in the control group.

CONCLUSIONS

The hUC-MSCs have definite therapeutic effects on acute radiation pneumonitis in rats.

摘要

目的

评估人脐带间充质干细胞（hUC-MSCs）对大鼠急性放射性肺炎的治疗效果。

方法

将40只大鼠随机分为对照组、辐射组、干细胞预防组、干细胞治疗组和泼尼松治疗组。除对照组外，其余大鼠均接受X射线照射以建立急性放射性肺炎损伤模型。预防组大鼠在辐射前24小时经尾静脉给予体外培养的hUC-MSCs（1×10(6)个细胞/千克体重），治疗组大鼠在辐射后24小时进行相同给药。辐射后24小时，辐射组大鼠给予0.4毫升生理盐水，泼尼松组大鼠给予1毫克/千克泼尼松。辐射后72小时观察并处死所有大鼠，检测肺组织学变化。