间充质干细胞注射大网膜补丁更有效地促进肠穿孔动物模型中的伤口愈合。
Mesenchymal Stem Cell-injected Omental Patch More Effective Promoting Wound Healing in Bowel Perforation Animal Model.
机构信息
Department of Surgery, Sultan Agung Islamic University, Semarang, Indonesia.
Stem Cell and Cancer Research, Sultan Agung Islamic University, Semarang, Indonesia.
出版信息
Med Arch. 2020 Oct;74(5):332-336. doi: 10.5455/medarh.2020.74.332-336.
INTRODUCTION
Bowel perforation (BP) occurs as the complication of many gastrointestinal problems. Omental patch (OP) is one of the methods to place omentum flaps in the perforated area. Mesenchymal stem cells (MSCs) may increase regeneration process in all tissues.
AIM
to demonstrate the role of MSC in accelerating of wound healing process by analyzing fibroblast and collagen appearance in perforated bowel conditions.
METHODS
Using a BP rabbit model, 18 rabbit were randomly assigned into three groups: combination of umbilical cord (UC)-MSCs injection and OP (T1), OP only (T2) and vehicle control (Veh). Hematoxylin-eosin staining and Masson's trichrome staining were performed to analyze the level of fibroblast and collagen. Wound length were measured using standardized caliper.
RESULTS
The study showed a significant (P<0.05) increase of fibroblast and collagen amount on T1 and T2, in which T1 was higher than T2. This result was also followed by the decrease of wound length.
CONCLUSION
The combination of MSCs and OP-sutured in perforated bowel are better to accelerate wound healing than OP only in BP cases.
简介
肠穿孔（BP）是许多胃肠道问题的并发症。大网膜贴片（OP）是将大网膜瓣放置在穿孔区域的方法之一。间充质干细胞（MSCs）可能会加速所有组织的再生过程。
目的
通过分析穿孔肠情况下成纤维细胞和胶原的外观，证明 MSC 在加速伤口愈合过程中的作用。
方法
使用 BP 兔模型，将 18 只兔子随机分为三组：脐带（UC）-MSCs 注射和 OP 的组合（T1）、仅 OP（T2）和载体对照（Veh）。进行苏木精-伊红染色和 Masson 三色染色以分析成纤维细胞和胶原的水平。使用标准化卡尺测量伤口长度。
结果
研究表明 T1 和 T2 中成纤维细胞和胶原数量显著增加（P<0.05），其中 T1 高于 T2。这一结果还伴随着伤口长度的减少。
结论
在 BP 病例中，与仅 OP 相比，OP 缝合的 MSC 和 MSCs 的组合更能加速伤口愈合。
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