Department of Paediatrics, Medical University of Vienna, Währinger Gürtel 18, 1090 Vienna, Austria.
Core Unit of Biomedical Research, Division of Laboratory Animal Science and Genetics, Medical University of Vienna, Brauhausgasse 34, A-2325 Himberg, Austria.
Behav Brain Res. 2014 May 1;264:1-8. doi: 10.1016/j.bbr.2014.01.042. Epub 2014 Feb 4.
Although a large series of reports on monoamine receptor (MAR) biochemistry and pharmacology in aging are available, work on MAR complexes rather than subunits is limited. It was the aim of the study to determine MAR complexes in hippocampi of three different age groups (3-12 and 18 months) in the mouse and to link MAR changes to spatial memory retrieval in the Morris water maze (MWM). MAR complexes were separated by blue native electrophoresis. Immunohistochemistry was performed in order to show the pattern of dopamine receptors and its colocalizations. D1R, D2R and 5-HT7R containing receptor complex levels were decreasing with age while 5-HT1AR-containing complex levels were increasing with age. D1R, 5-HT7R and 5-HT1AR were significantly correlating with the time spent in the target quadrant, representing retrieval in the MWM. D1R and D2R immunoreactivity was decreasing in an area-dependent pattern and D1R and D2R were colocalized. Individual monoamine receptors are linked to spatial memory retrieval and are modulated by age. The findings are relevant for interpretation of previous and design of future work on brain receptors, spatial memory and aging.
尽管有大量关于单胺受体 (MAR) 生物化学和药理学在衰老过程中的报告，但对 MAR 复合物而非亚基的研究有限。本研究旨在确定小鼠海马体中三个不同年龄组（3-12 个月和 18 个月）的 MAR 复合物，并将 MAR 变化与 Morris 水迷宫 (MWM) 中的空间记忆检索联系起来。MAR 复合物通过蓝色-native 电泳分离。进行免疫组织化学染色以显示多巴胺受体及其共定位模式。D1R、D2R 和 5-HT7R 受体复合物的水平随年龄增长而降低，而 5-HT1AR 受体复合物的水平随年龄增长而升高。D1R、5-HT7R 和 5-HT1AR 与在目标象限花费的时间呈显著相关，代表在 MWM 中的检索。D1R 和 D2R 的免疫反应性呈区域依赖性降低，且 D1R 和 D2R 共定位。个体单胺受体与空间记忆检索有关，并受年龄调节。这些发现对于解释以前的研究和设计未来关于大脑受体、空间记忆和衰老的工作具有重要意义。
