Benjamin D C, Weigle W O
J Exp Med. 1970 Jul 1;132(1):66-76. doi: 10.1084/jem.132.1.66.
Rabbits made unresponsive to BSA at birth were given two courses of immunization with various cross-reacting albumins at 3 months of age. Normal control rabbits, of equivalent age and weight, were similarly immunized. Sera obtained 7 days after the last injection were assayed for binding and precipitating antibody to six albumins and for their avidity for BSA. No significant differences were found between unresponsive and normal rabbits in the amount of antibody reacting with any of the six albumins used. This was the case regardless which albumin was used to terminate the unresponsive state. Avidity differences were seen and seemed to depend on the antigen used and not on the immunological status of the animal. The simultaneous injection of small amounts of BSA inhibited the termination of unresponsiveness. These results were discussed in the light of the more recent theories of the termination of unresponsiveness and of antibody formation.
出生时对牛血清白蛋白（BSA）无反应的兔子在3月龄时用各种交叉反应白蛋白进行了两个疗程的免疫接种。选取年龄和体重相当的正常对照兔子进行同样的免疫接种。在最后一次注射7天后采集血清，检测针对六种白蛋白的结合抗体和沉淀抗体以及它们对BSA的亲和力。在与所使用的六种白蛋白中的任何一种发生反应的抗体量方面，无反应兔子和正常兔子之间未发现显著差异。无论使用哪种白蛋白来终止无反应状态，情况都是如此。观察到了亲和力差异，而且似乎取决于所使用的抗原，而不是动物的免疫状态。同时注射少量BSA会抑制无反应状态的终止。根据关于无反应状态终止和抗体形成的最新理论对这些结果进行了讨论。
