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基于 mcrA 基因序列的培养独立方法研究韩牛(Bos taurus coreanae)瘤胃液、直肠粪便和牛舍粪肥中产甲烷古菌的多样性。

Methanogenic archaea diversity in Hanwoo (Bos taurus coreanae) rumen fluid, rectal dung, and barn floor manure using a culture-independent method based on mcrA gene sequences.

机构信息

Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 660-701, Republic of Korea.

Departement of Food Science, Gyeongnam National University of Science and Technology, Jinju 660-758, Republic of Korea.

出版信息

Anaerobe. 2014 Jun;27:77-81. doi: 10.1016/j.anaerobe.2014.01.008. Epub 2014 Feb 7.

Abstract

The diversity of methanogenic archaea associated with Korean Hanwoo cattle was analyzed using mcrA gene sequences from samples of rumen fluid (RF), rectal dung (RD), and barn floor manure (BFM). The predominant species were Methanobrevibacter ruminantium in RF and BFM(63.6% and 62.4%, respectively) and Methanocorpusculum labreanum in RD (53.2%).

摘要

采用瘤胃液(RF)、直肠粪便(RD)和牛舍地板粪肥(BFM)样本中的 mcrA 基因序列,分析与韩国韩牛相关的产甲烷古菌的多样性。RF 和 BFM 中的主要种为瘤胃甲烷短杆菌(Methanobrevibacter ruminantium)(分别为 63.6%和 62.4%),RD 中的主要种为巴氏甲烷八叠球菌(Methanocorpusculum labreanum)(53.2%)。

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