Dong Minghua, Wu Yan, Li Qiumin, Tian Guangliang, Yang Bin, Li Yingjuan, Zhang Lijuan, Wang Yongxia, Xiao Wei, Yin Fang, Zhao Xingling, Zhang Wudi, Cui Xiaolong
Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, China,
Curr Microbiol. 2015 May;70(5):679-84. doi: 10.1007/s00284-015-0775-0. Epub 2015 Jan 10.
Understanding of the microbial community structures of the biogas digesters in different climatic regions can help improve the methane production in the fermentation process. The methanogenic archaeal diversity in four rural biogas digesters (BNA, JSA, LJA, and XGA) was investigated by a culture-independent rRNA approach in different climatic regions in Yunnan. Community structure composed of 711 clones in the all libraries. A total of 33 operational taxonomic units (OTUs) were detected, and major groups of methanogens were the orders Methanosarcinales and Methanomicrobiales. 63.2 % of all archaeal OTUs belong to the order Methanosarcinales which mostly contain acetotrophic methanogens. Methanomicrobiales (19.5 % in all OTUs) were detected in considerable number. Additionally, there were minor rates of uncultured archaea. The principal component analysis indicated that the genus Methanosaeta was mainly affected by the fermentation temperatures.
了解不同气候区域沼气池的微生物群落结构有助于提高发酵过程中的甲烷产量。通过非培养rRNA方法对云南不同气候区域的四个农村沼气池（BNA、JSA、LJA和XGA）中的产甲烷古菌多样性进行了研究。所有文库中由711个克隆组成群落结构。共检测到33个操作分类单元（OTU），产甲烷菌的主要类群是甲烷八叠球菌目和甲烷微菌目。所有古菌OTU的63.2%属于甲烷八叠球菌目，其中大多包含乙酸营养型产甲烷菌。检测到相当数量的甲烷微菌目（占所有OTU的19.5%）。此外，未培养古菌的比例较小。主成分分析表明，甲烷鬃菌属主要受发酵温度的影响。
