Kogut Igor, Roop Dennis R, Bilousova Ganna
Charles C. Gates Center for Regenerative Medicine and Stem Cell Biology, University of Colorado, Anschutz Medical Campus, Mail Stop 8320, 6511, Aurora, CO, 80045, USA.
Methods Mol Biol. 2014;1195:1-12. doi: 10.1007/7651_2013_64.
Direct reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) provides an opportunity to develop novel personalized treatment options for numerous diseases and to advance current approaches for cell-based drug discoveries and disease modeling. The ability to differentiate iPSCs into relevant cell types is an important prerequisite for the successful development of iPSC-based treatment and modeling strategies. Here, we describe a protocol for the efficient differentiation of human iPSCs into functional keratinocytes. The protocol employs treating iPSCs with retinoic acid and bone-morphogenetic protein-4 to induce differentiation toward a keratinocyte lineage, which is then followed by the growth of differentiated iPSCs on collagen type I- and collagen type IV-coated dishes to enrich for iPSC-derived keratinocytes.
将体细胞直接重编程为诱导多能干细胞（iPSC）为开发针对多种疾病的新型个性化治疗方案以及推进基于细胞的药物发现和疾病建模的现有方法提供了契机。将iPSC分化为相关细胞类型的能力是成功开发基于iPSC的治疗和建模策略的重要前提。在此，我们描述了一种将人iPSC高效分化为功能性角质形成细胞的方案。该方案采用视黄酸和骨形态发生蛋白-4处理iPSC以诱导其向角质形成细胞谱系分化，随后将分化的iPSC在包被有I型胶原和IV型胶原的培养皿上生长，以富集iPSC来源的角质形成细胞。
