将人诱导多能干细胞分化为角质形成细胞谱系。
Differentiation of human induced pluripotent stem cells into a keratinocyte lineage.
作者信息
Kogut Igor, Roop Dennis R, Bilousova Ganna
机构信息
Charles C. Gates Center for Regenerative Medicine and Stem Cell Biology, University of Colorado, Anschutz Medical Campus, Mail Stop 8320, 6511, Aurora, CO, 80045, USA.
出版信息
Methods Mol Biol. 2014;1195:1-12. doi: 10.1007/7651_2013_64.
Abstract
Direct reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) provides an opportunity to develop novel personalized treatment options for numerous diseases and to advance current approaches for cell-based drug discoveries and disease modeling. The ability to differentiate iPSCs into relevant cell types is an important prerequisite for the successful development of iPSC-based treatment and modeling strategies. Here, we describe a protocol for the efficient differentiation of human iPSCs into functional keratinocytes. The protocol employs treating iPSCs with retinoic acid and bone-morphogenetic protein-4 to induce differentiation toward a keratinocyte lineage, which is then followed by the growth of differentiated iPSCs on collagen type I- and collagen type IV-coated dishes to enrich for iPSC-derived keratinocytes.
摘要
将体细胞直接重编程为诱导多能干细胞（iPSC）为开发针对多种疾病的新型个性化治疗方案以及推进基于细胞的药物发现和疾病建模的现有方法提供了契机。将iPSC分化为相关细胞类型的能力是成功开发基于iPSC的治疗和建模策略的重要前提。在此，我们描述了一种将人iPSC高效分化为功能性角质形成细胞的方案。该方案采用视黄酸和骨形态发生蛋白-4处理iPSC以诱导其向角质形成细胞谱系分化，随后将分化的iPSC在包被有I型胶原和IV型胶原的培养皿上生长，以富集iPSC来源的角质形成细胞。
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本文引用的文献
1
Feeder-free derivation of human induced pluripotent stem cells with messenger RNA.无饲养层条件下人诱导多能干细胞的信使 RNA 衍生。
Sci Rep. 2012;2:657. doi: 10.1038/srep00657. Epub 2012 Sep 14.
2
Cellular reprogramming--lowering gravity on Waddington's epigenetic landscape.细胞重编程——降低 Waddington 表观遗传景观中的重力。
J Cell Sci. 2012 Jun 1;125(Pt 11):2553-60. doi: 10.1242/jcs.084822. Epub 2012 Jun 26.
3
Generation of keratinocytes from normal and recessive dystrophic epidermolysis bullosa-induced pluripotent stem cells.从正常和隐性营养不良性大疱性表皮松解症诱导多能干细胞中生成角质形成细胞。
Proc Natl Acad Sci U S A. 2011 May 24;108(21):8797-802. doi: 10.1073/pnas.1100332108. Epub 2011 May 9.
4
Differentiation of mouse induced pluripotent stem cells into a multipotent keratinocyte lineage.将小鼠诱导多能干细胞分化为多能角质细胞谱系。
J Invest Dermatol. 2011 Apr;131(4):857-64. doi: 10.1038/jid.2010.364. Epub 2010 Dec 9.
5
Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA.利用合成修饰 mRNA 高效重编程人类细胞为多能性干细胞并进行定向分化。
Cell Stem Cell. 2010 Nov 5;7(5):618-30. doi: 10.1016/j.stem.2010.08.012. Epub 2010 Sep 30.
6
A fresh look at iPS cells.对诱导多能干细胞的全新审视。
Cell. 2009 Apr 3;137(1):13-7. doi: 10.1016/j.cell.2009.03.034.
7
The promise of human induced pluripotent stem cells for research and therapy.人类诱导多能干细胞在研究和治疗方面的前景。
Nat Rev Mol Cell Biol. 2008 Sep;9(9):725-9. doi: 10.1038/nrm2466.
8
Strategies and new developments in the generation of patient-specific pluripotent stem cells.生成患者特异性多能干细胞的策略与新进展。
Cell Stem Cell. 2007 Jun 7;1(1):39-49. doi: 10.1016/j.stem.2007.05.012.
9
Induction of pluripotent stem cells from adult human fibroblasts by defined factors.通过特定因子将成人成纤维细胞诱导为多能干细胞。
Cell. 2007 Nov 30;131(5):861-72. doi: 10.1016/j.cell.2007.11.019.
10
Induced pluripotent stem cell lines derived from human somatic cells.源自人类体细胞的诱导多能干细胞系。
Science. 2007 Dec 21;318(5858):1917-20. doi: 10.1126/science.1151526. Epub 2007 Nov 20.
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