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合成基因的设计

The design of synthetic genes.

作者信息

Presnell S R, Benner S A

机构信息

Laboratory for Organic Chemistry, ETH-Zentrum, Zurich, Switzerland.

出版信息

Nucleic Acids Res. 1988 Mar 11;16(5):1693-702. doi: 10.1093/nar/16.5.1693.

DOI:10.1093/nar/16.5.1693
PMID:2451218
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC338164/
Abstract

Computer programs are described that aid in the design of synthetic genes coding for proteins that are targets of a research program in site directed mutagenesis. These programs "reverse-translate" protein sequences into general nucleic acid sequences (those where codons have not yet been selected), map restriction sites into general DNA sequences, identify points in the synthetic gene where unique restriction sites can be introduced, and assist in the design of genes coding for hybrids and evolutionary intermediates between homologous proteins. Application of these programs therefore facilitates the use of modular mutagenesis to create variants of proteins, and the implementation of evolutionary guidance as a strategy for selecting mutants.

摘要

本文描述了一些计算机程序，这些程序有助于设计编码蛋白质的合成基因，而这些蛋白质是定点诱变研究项目的目标。这些程序将蛋白质序列“反向翻译”为通用核酸序列（即尚未选择密码子的序列），将限制性酶切位点映射到通用DNA序列中，识别合成基因中可引入独特限制性酶切位点的位置，并协助设计编码同源蛋白质之间的杂交体和进化中间体的基因。因此，这些程序的应用有助于利用模块化诱变来创建蛋白质变体，并将进化指导作为一种选择突变体的策略加以实施。

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本文引用的文献

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Synthesis of the human insulin gene. Part III. Chemical synthesis of 5'-phosphomonoester group containing deoxyribooligonucleotides by the modified phosphotriester method. Its application in the synthesis of seventeen fragments constituting human insulin C-chain DNA.人胰岛素基因的合成。第三部分。用改进的磷酸三酯法化学合成含5'-磷酸单酯基团的脱氧核糖寡核苷酸。其在构成人胰岛素C链DNA的17个片段合成中的应用。
Nucleic Acids Res. 1980 Dec 11;8(23):5753-65. doi: 10.1093/nar/8.23.5753.
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Codon usage in bacteria: correlation with gene expressivity.细菌中的密码子使用：与基因表达能力的相关性
Nucleic Acids Res. 1982 Nov 25;10(22):7055-74. doi: 10.1093/nar/10.22.7055.
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Total synthesis and cloning of a gene coding for the ribonuclease S protein.核糖核酸酶S蛋白编码基因的全合成与克隆
Science. 1984 Mar 23;223(4642):1299-301. doi: 10.1126/science.6322300.
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Chemical synthesis of a human interferon-alpha 2 gene and its expression in Escherichia coli.人α-2干扰素基因的化学合成及其在大肠杆菌中的表达。
Nucleic Acids Res. 1983 Sep 24;11(18):6419-35. doi: 10.1093/nar/11.18.6419.
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Expression in Escherichia coli of chemically synthesized gene for the human immune interferon.人免疫干扰素化学合成基因在大肠杆菌中的表达
Nucleic Acids Res. 1983 Mar 25;11(6):1707-23. doi: 10.1093/nar/11.6.1707.
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Production in Escherichia coli of biologically active secretin, a gastrointestinal hormone.在大肠杆菌中生产具有生物活性的促胰液素（一种胃肠激素）。
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2475-9. doi: 10.1073/pnas.79.8.2475.
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Total synthesis of a human leukocyte interferon gene.人白细胞干扰素基因的全合成
Nature. 1981 Aug 20;292(5825):756-62. doi: 10.1038/292756a0.
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Synthesis of a gene for human growth hormone and its expression in Escherichia coli.人生长激素基因的合成及其在大肠杆菌中的表达。
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Effects of site-specific amino acid modification on protein interactions and biological function.
Annu Rev Biochem. 1985;54:597-629. doi: 10.1146/annurev.bi.54.070185.003121.
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Chemical synthesis of a gene encoding the human complement fragment C5a and its expression in Escherichia coli.
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3543-7. doi: 10.1073/pnas.82.11.3543.

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