人胰岛素基因的合成。第三部分。用改进的磷酸三酯法化学合成含5'-磷酸单酯基团的脱氧核糖寡核苷酸。其在构成人胰岛素C链DNA的17个片段合成中的应用。
Synthesis of the human insulin gene. Part III. Chemical synthesis of 5'-phosphomonoester group containing deoxyribooligonucleotides by the modified phosphotriester method. Its application in the synthesis of seventeen fragments constituting human insulin C-chain DNA.
作者信息
Hsiung H M, Sung W L, Brousseau R, Wu R, Narang S A
出版信息
Nucleic Acids Res. 1980 Dec 11;8(23):5753-65. doi: 10.1093/nar/8.23.5753.
A method for phosphorylating a protected deoxyribooligonucleotide containing phosphotriester linkages is described. The modified phosphotriester method of chemical synthesis is further refined in terms of (i) better final deblocking conditions and (ii) new chromatography solvent systems containing acetone-water-ethyl acetate to yield pure oligomers. The effectiveness of these improvements has been demonstrated in the rapid and efficient synthesis of seventeen fragments constituting the sequence of human insulin C-chain DNA.
描述了一种对含有磷酸三酯键的受保护脱氧核糖寡核苷酸进行磷酸化的方法。化学合成的改进磷酸三酯方法在以下方面得到了进一步完善：（i）更好的最终脱保护条件，以及（ii）含有丙酮 - 水 - 乙酸乙酯的新型色谱溶剂系统，以产生纯的寡聚物。这些改进的有效性已在快速高效合成构成人胰岛素C链DNA序列的17个片段中得到证明。
Zotero 插件