Rubin K, Terracio L, Rönnstrand L, Heldin C H, Klareskog L
Department of Medical and Physiological Chemistry, University of Uppsala, Sweden.
Scand J Immunol. 1988 Mar;27(3):285-94. doi: 10.1111/j.1365-3083.1988.tb02349.x.
The tissue distribution of the receptor for platelet-derived growth factor (PDGF) was investigated by immunohistochemistry on frozen sections from normal and inflamed synovial tissue using monoclonal antibodies to the receptor. Non-inflamed synovial tissue showed no staining, indicating that PDGF receptor expression is low or absent in normal tissue. In contrast, tissue from synovitis with prominent neovascularization showed a strong staining in the tunica media of the proliferating blood vessels as well as on connective tissue cells in the stroma. Tissue from synovitis with prominent proliferation of synovial lining showed intense staining for PDGF receptors in fibroblast-like cells of the lining and a less intense staining on vascular and connective tissue cells deeper in the stroma. Staining for PDGF receptors was also intense in the pannus tissue close to infiltrated bone and cartilage. In all these forms of synovitis, PDGF receptor staining was associated with increased HLA-DR staining and infiltration of macrophages and T lymphocytes. The finding that PDGF receptor expression is induced in conjunction with the chronic synovial inflammation associated with rheumatoid arthritis and some other forms of arthritides suggests that PDGF may play a role in the stimulation of mesenchymal cell proliferation that often accompanies chronic inflammatory disease.
利用抗血小板衍生生长因子（PDGF）受体的单克隆抗体，通过免疫组织化学方法对正常和炎症滑膜组织的冰冻切片进行研究，以探讨该受体的组织分布情况。未发炎的滑膜组织未显示染色，表明正常组织中PDGF受体表达较低或不存在。相比之下，具有明显新生血管形成的滑膜炎组织在增生血管的中膜以及基质中的结缔组织细胞上显示出强烈染色。具有明显滑膜衬里增生的滑膜炎组织在衬里的成纤维细胞样细胞中显示出对PDGF受体的强烈染色，而在基质深处的血管和结缔组织细胞上染色较弱。靠近浸润骨和软骨的血管翳组织中PDGF受体染色也很强烈。在所有这些形式的滑膜炎中，PDGF受体染色与HLA - DR染色增加以及巨噬细胞和T淋巴细胞浸润相关。PDGF受体表达在与类风湿性关节炎和其他一些关节炎相关的慢性滑膜炎症中被诱导这一发现表明，PDGF可能在刺激通常伴随慢性炎症疾病的间充质细胞增殖中发挥作用。
