Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States.
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States.
Curr Opin Chem Biol. 2014 Apr;19:67-75. doi: 10.1016/j.cbpa.2014.01.006. Epub 2014 Feb 8.
Metalloenzymes are among the major targets of protein design and engineering efforts aimed at attaining novel and efficient catalysis for biochemical transformation and biomedical applications, due to the diversity of functions imparted by the metallo-cofactors along with the versatility of the protein environment. Naturally evolved protein scaffolds can often serve as robust foundations for sustaining artificial active sites constructed by rational design, directed evolution, or a combination of the two strategies. Accumulated knowledge of structure-function relationship and advancement of tools such as computational algorithms and unnatural amino acids incorporation all contribute to the design of better metalloenzymes with catalytic properties approaching the needs of practical applications.
金属酶是蛋白质设计和工程努力的主要目标之一，旨在为生化转化和生物医学应用获得新颖和高效的催化作用，这是由于金属辅因子赋予的功能多样性以及蛋白质环境的多功能性。自然进化的蛋白质支架通常可以作为合理设计、定向进化或这两种策略组合构建的人工活性位点的稳健基础。结构-功能关系的积累知识和计算算法工具以及非天然氨基酸掺入等工具的进步都有助于设计具有接近实际应用需求的催化特性的更好的金属酶。
