Peters K, Serup J
Department of Dermatology, Gentofte Hospital, Copenhagen, Denmark.
Acta Derm Venereol. 1987;67(6):491-5.
Silicone rubber replica of patch test reactions were obtained from 12 patients with allergic contact dermatitis. 85 positive or doubtful reactions to allergens included in a standard battery were studied. The number of papulo-vesicles and sweat pores as seen in the replica were counted under a stereomicroscope. The papulo-vesicular count increased significantly in relation to clinical reading of the reactions of increasing strength. The number of sweat pores of positive reactions was decreased. This was probably due to cutaneous oedema with compression of sweat gland ducts. Papulo-vesicles are also consequences of the inflammatory oedema. The replica method was concluded to be a useful tool for the grading of allergic patch test reactions. It has the virtues of reproducibility, simplicity and low cost, and it can be evaluated under standardized laboratory conditions. Replica may also be the basis for more sophisticated methods of quantitation such as computer-aided image analysis.
从12例过敏性接触性皮炎患者身上获取了斑贴试验反应的硅橡胶复制品。研究了对标准系列中包含的变应原的85例阳性或可疑反应。在体视显微镜下计数复制品中可见的丘疹水疱数量和汗孔数量。丘疹水疱计数随着反应强度增加的临床读数而显著增加。阳性反应的汗孔数量减少。这可能是由于皮肤水肿压迫汗腺导管所致。丘疹水疱也是炎症性水肿的结果。得出结论，复制品方法是过敏性斑贴试验反应分级的一种有用工具。它具有可重复性、简单性和低成本的优点，并且可以在标准化实验室条件下进行评估。复制品也可能是更复杂的定量方法（如计算机辅助图像分析）的基础。
