鱼精蛋白对豚鼠肠系膜肥大细胞的非细胞毒性刺激作用

Non cytotoxic guinea-pig mesenteric mast cell stimulation by protamine.

作者信息

Augusto C, Lunardi L O, Vugman I

机构信息

Department of Morphology, Faculty of Medicine, Ribeirão Preto, Brazil.

出版信息

Agents Actions. 1987 Dec;22(3-4):185-8. doi: 10.1007/BF02009044.

DOI:10.1007/BF02009044

PMID:2451398

Abstract

Protamine stimulates guinea-mesenteric mast cells in a concentration-dependent manner, both histamine release and mast cell degranulation being correlated. Mast cell stimulation is blocked by 2,4-DNP (0.03 mM), low (0 degrees C) and high (45 degrees C) temperature. The inhibitory effect by 2,4-DNP is reversed by glucose (5.0 mM), while incubation at 37 degrees reverses that by low and high temperature. Lack of calcium from the incubation medium does not influence mast cell stimulation by protamine. However calcium chelation with EDTA (2.0 mM) or EGTA (2.0 mM) blocks mast cell stimulation. Addition of calcium (0.9 mM) reverses this inhibition. These observations indicate that guinea-pig mast cell stimulation by protamine is a nonlytic, energy and calcium dependent process, similar to anaphylaxis, but different from that of other basic compounds which induce mast cell lysis.

摘要

鱼精蛋白以浓度依赖的方式刺激豚鼠肠系膜肥大细胞，组胺释放和肥大细胞脱颗粒呈正相关。肥大细胞刺激被2,4 -二硝基苯酚（0.03 mM）、低温（0℃）和高温（45℃）阻断。2,4 -二硝基苯酚的抑制作用可被葡萄糖（5.0 mM）逆转，而在37℃孵育可逆转低温和高温的抑制作用。孵育培养基中缺乏钙不影响鱼精蛋白对肥大细胞的刺激。然而，用乙二胺四乙酸（EDTA，2.0 mM）或乙二醇双乙醚二胺四乙酸（EGTA，2.0 mM）螯合钙可阻断肥大细胞刺激。添加钙（0.9 mM）可逆转这种抑制作用。这些观察结果表明，鱼精蛋白对豚鼠肥大细胞的刺激是一个非溶细胞的、能量和钙依赖性过程，类似于过敏反应，但不同于其他诱导肥大细胞裂解的碱性化合物。