莱茵衣藻的两种钙依赖性蛋白激酶受营养饥饿的转录调控。
Two calcium-dependent protein kinases from Chlamydomonas reinhardtii are transcriptionally regulated by nutrient starvation.
作者信息
Motiwalla Mustafa J, Sequeira Marilyn P, D'Souza Jacinta S
机构信息
UM-DAE Centre for Excellence in Basic Sciences; Kalina Campus; Santacruz (E); Mumbai-400098, India.
出版信息
Plant Signal Behav. 2014;9(1):e27969. doi: 10.4161/psb.27969. Epub 2014 Jan 29.
Abstract
We report here, the transcriptional regulation of 2 Calcium Dependent Protein Kinases in response to nutrient starvation of Chlamydomonas reinhardtii vegetative cells. The CDPK proteins, CDPK1 and CDPK3; share 53% identity among themselves, a maximum of 57% and 52% to higher plants respectively and 42% to apicomplexan protozoans. We expressed a CDPK1-GFP fusion protein in the C. reinhardtii vegetative cells and showed its distribution both in the cell body and the membrane-matrix fraction of the flagella. The fusion protein exhibits mobility shift in the presence of Ca (2+), confirming its Ca (2+)-binding properties. To the best of our knowledge, this is the first report of transcriptional regulation of CDPKs from a unicellular chlorophyte in response to nutrient starvation namely acetate (A), phosphorus (P), and nitrogen (N).
摘要
我们在此报告莱茵衣藻营养细胞在营养饥饿状态下，2种钙依赖蛋白激酶的转录调控情况。钙依赖蛋白激酶（CDPK）蛋白CDPK1和CDPK3彼此间有53%的一致性，与高等植物的一致性最高分别为57%和52%，与顶复门原生动物的一致性为42%。我们在莱茵衣藻营养细胞中表达了一种CDPK1 - GFP融合蛋白，并展示了其在细胞体和鞭毛膜 - 基质部分的分布情况。该融合蛋白在Ca（2+）存在时表现出迁移率变化，证实了其Ca（2+）结合特性。据我们所知，这是关于单细胞绿藻中CDPKs响应营养饥饿（即乙酸盐（A）、磷（P）和氮（N））的转录调控的首次报道。
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