使用膜结合合成重叠肽进行表位作图。
Epitope mapping with membrane-bound synthetic overlapping peptides.
作者信息
Midoro-Horiuti Terumi, Goldblum Randall M
机构信息
Department of Pediatrics, University of Texas Medical Branch, Galveston, TX, USA.
出版信息
Methods Mol Biol. 2014;1131:421-6. doi: 10.1007/978-1-62703-992-5_25.
Abstract
Epitope mapping with synthetic overlapping peptides is used for identifying epitopes of monoclonal antibodies (mAbs) and antibodies from patient sera (Midoro-Horiuti et al. Mol Immunol 43:509-518, 2006; Ivanciuc et al. J Agric Food Chem 51:4830-4837, 2003; Midoro-Horiuti et al. Mol Immunol 40:555-562, 2003; Wang et al. J Allergy Clin Immunol 125:695-702, 702.e1-702.e6, 2010). When the mAbs recognize epitopes that are also recognized by patients of interest, they may be useful as surrogates for patient antibodies.
摘要
使用合成重叠肽进行表位作图可用于鉴定单克隆抗体（mAb）和患者血清中的抗体表位（Midoro-Horiuti等人，《分子免疫学》43:509 - 518，2006年；Ivanciuc等人，《农业与食品化学杂志》51:4830 - 4837，2003年；Midoro-Horiuti等人，《分子免疫学》40:555 - 562，2003年；Wang等人，《过敏与临床免疫学杂志》125:695 - 702，702.e1 - 702.e6，2010年）。当单克隆抗体识别的表位也被感兴趣的患者识别时，它们可能可用作患者抗体的替代物。
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