Razzeca K J, Kim S Y, Rouse R V
Department of Pathology, Stanford University, California 94305.
Cell Immunol. 1988 Apr 1;112(2):442-8. doi: 10.1016/0008-8749(88)90314-0.
We have employed two-color staining with monoclonal anti-T-cell markers and a broadly reactive, nonbinding site monoclonal anti-idiotype to permit direct visualization of idiotype-bearing T cells in mouse lymph nodes following immunization with phosphorylcholine. Double positive cells peak in incidence on Days 8 to 12, as 17% of total idiotype+ cells but as only 0.7% of T cells. Such cells are antigen-specific, appearing in peripheral lymph nodes only following phosphorylcholine challenge. While Lyt-2+ and L3T4+ subsets are represented and both subsets reexpress the idiotypic determinant following its enzymatic removal, the L3T4+ subset represents the majority of idiotype+Thy-1+ cells. These findings raise the possibility that antigen-specific receptors on T and B cells, encoded by entirely different genetic information, may exhibit similarities in tertiary structure in portions of the molecules not directly involved in antigen binding. This is the first determination of the phenotype and chronology of appearance of idiotype-bearing normal T cells following immunization. It is consistent with previous reports of functionally defined idiotype-bearing T cells and provides direct support for the existence of such cells.
我们采用了双色染色法，使用单克隆抗T细胞标志物和一种具有广泛反应性、非结合位点的单克隆抗独特型抗体，以便在用磷酸胆碱免疫后直接观察小鼠淋巴结中携带独特型的T细胞。双阳性细胞的发生率在第8至12天达到峰值，占独特型阳性细胞总数的17%，但仅占T细胞的0.7%。这些细胞具有抗原特异性，仅在磷酸胆碱攻击后出现在外周淋巴结中。虽然Lyt-2阳性和L3T4阳性亚群均有出现，且在酶去除独特型决定簇后两个亚群都重新表达独特型决定簇，但L3T4阳性亚群占独特型阳性Thy-1阳性细胞的大多数。这些发现增加了一种可能性，即由完全不同的遗传信息编码的T细胞和B细胞上的抗原特异性受体，在分子中不直接参与抗原结合的部分可能在三级结构上表现出相似性。这是首次确定免疫后携带独特型的正常T细胞的表型和出现时间顺序。它与先前关于功能定义的携带独特型T细胞的报道一致，并为这类细胞的存在提供了直接支持。
