Bottomly K, Mathieson B J, Mosier D E
J Exp Med. 1978 Nov 1;148(5):1216-27. doi: 10.1084/jem.148.5.1216.
An adoptive secondary antibody response to phosphorylcholine (PC) can be generated by the transfer of keyhole limpet hemocyanin (KLH)-primed T cells, PC-bovine gamma globulin-primed B cells, and PC-KLH into irradiated syngeneic BALB/c mice. If the KLH-primed T-cell donors were pretreated with anti-idiotype antibodies directed against the BALB/c PC-binding myeloma TEPC 15, their T cells were unable to collaborate effectively with PC-primed B cells; moreover, they could suppress the helper activity of T cells from normal mice for the PC-KLH response. The Ly phenotype of these T cells was found to be Ly 1-, 2+. The specificity of the suppressor T-cell population induced by anti-T15 treatment appears to be both for idiotype (hapten) and carrier, since the suppressor T cells fail to interfere with the antibody response to PC on a heterologous carrier, nor do they suppress the response to trinitrophenol-KLH.
通过将钥孔戚血蓝蛋白(KLH)致敏的T细胞、磷酸胆碱 - 牛γ球蛋白致敏的B细胞和磷酸胆碱 - KLH转移至经照射的同基因BALB/c小鼠体内,可产生对磷酸胆碱(PC)的过继性二次抗体应答。如果用针对BALB/c PC结合骨髓瘤TEPC 15的抗独特型抗体预处理KLH致敏的T细胞供体,其T细胞就无法与PC致敏的B细胞有效协作;此外,它们还能抑制正常小鼠T细胞对PC - KLH应答的辅助活性。发现这些T细胞的Ly表型为Ly 1 -、2 +。抗T15处理诱导的抑制性T细胞群体的特异性似乎针对独特型(半抗原)和载体,因为抑制性T细胞不会干扰对异源载体上PC的抗体应答,也不会抑制对三硝基苯酚 - KLH的应答。