Passelecq B, De Bo M, Huber C, Gennart J P, Bernard A, Lauwerys R
Unité de Toxicologie Industrielle et Médicale, Université Catholique de Louvain, Brussels, Belgium.
J Immunol Methods. 1988 Apr 22;109(1):69-74. doi: 10.1016/0022-1759(88)90443-7.
A latex particle immunoassay has been developed for the determination of serum alpha-fetoprotein. The assay consists of incubating the serum sample for 30 min at 50 degrees C with latex particles coated with anti-alpha-fetoprotein immunoglobulin, then quantifying the remaining unagglutinated particles with an optical particle counter. The assay is fully automated with a sampling rate of 40/h. The interference from rheumatoid factor is eliminated by precipitation with polyethylene glycol (7% final concentration). The alpha-fetoprotein standard curve, prepared in a human serum matrix, extends from 0.5 to 32 micrograms/l. Because sera are diluted ten-fold, the limit of detection of the assay lies around 5 micrograms/l. Coefficients of variation ranged from 4 to 15% and the recovery of alpha-fetoprotein tested on eight normal sera and four sera containing rheumatoid factor averaged 102.2% (SD = 13.2). The correlation coefficient between latex immunoassay and radioimmunoassay, calculated from the assay of 138 samples was 0.98.
已开发出一种用于测定血清甲胎蛋白的乳胶颗粒免疫测定法。该测定法包括将血清样本与包被有抗甲胎蛋白免疫球蛋白的乳胶颗粒在50℃下孵育30分钟，然后用光学颗粒计数器对剩余未凝集的颗粒进行定量。该测定法完全自动化，采样率为每小时40次。通过用聚乙二醇（最终浓度为7%）沉淀消除类风湿因子的干扰。在人血清基质中制备的甲胎蛋白标准曲线范围为0.5至32微克/升。由于血清被稀释了10倍，该测定法的检测限约为5微克/升。变异系数范围为4%至15%，在8份正常血清和4份含类风湿因子的血清上测试的甲胎蛋白回收率平均为102.2%（标准差 = 13.2）。根据对138个样本的测定计算，乳胶免疫测定法与放射免疫测定法之间的相关系数为0.98。
