Bernard A, Lauwerys R
J Immunol Methods. 1984 Jul 6;71(2):141-7. doi: 10.1016/0022-1759(84)90060-7.
A sensitive immunoassay based on latex particle agglutination (latex immunoassay) has been developed for the determination of serum ferritin. Polystyrene particles (0.8 micron) to which anti-ferritin antibody is adsorbed are incubated with serum for 30 min with shaking or 60 min without shaking. The resulting agglutination is quantified by measuring the decrease of absorbance of the latex suspension at 360 nm. Pretreatment of the serum is required to prevent interference from complement, rheumatoid factor and chylomicrons. The lowest detectable concentration of ferritin in serum is about 5 micrograms/l. Within-runs and between-runs coefficients of variation, based on analysis of samples containing 14-820 micrograms/l ferritin, ranged from 3.6 to 13.8%. A correlation coefficient of 0.95 between latex immunoassay and radioimmunoassay was obtained for the determination of ferritin in 46 sera.
已开发出一种基于乳胶颗粒凝集的灵敏免疫测定法（乳胶免疫测定法）用于测定血清铁蛋白。将吸附有抗铁蛋白抗体的聚苯乙烯颗粒（0.8微米）与血清一起在振荡条件下孵育30分钟或在无振荡条件下孵育60分钟。通过测量乳胶悬浮液在360nm处吸光度的下降来对产生的凝集进行定量。血清需要进行预处理以防止补体、类风湿因子和乳糜微粒的干扰。血清中铁蛋白的最低可检测浓度约为5微克/升。基于对含有14 - 820微克/升铁蛋白的样品的分析，批内和批间变异系数范围为3.6%至13.8%。在对46份血清中的铁蛋白进行测定时，乳胶免疫测定法与放射免疫测定法之间的相关系数为0.95。
