Collet-Cassart D, Magnusson C G, Ratcliffe J G, Cambiaso C L, Masson P L
Clin Chem. 1981 Jan;27(1):64-7.
PACIA, a homogeneous non-radioimmunoassay, has been adapted to the determination of serum alpha 1-fetoprotein. This technique is based on the agglutination of latex particles coated with antibodies to the antigen to be determined. The agglutination is measured by using an optical cell counter designed to count blood cells, to determine the reduction in the number of non-agglutinated particles. Interferences by serum constituents are avoided by coating the particles with the F(ab')2-fragments of th immunoglobulin G fraction of the antiserum. The system is automated, with a sampling rate of 50/h and an incubation time of 26 min. Concentrations used in preparing the standard curve ranged from 1 to 50 microgram/L; analytical recoveries were 93.5 to 98.4%; the correlation coefficient of PACIA with radioimmunoassay, calculated from results on 127 samples, was 0.98; maximum within- and between-assay CVs were 7.4% and 9.6%, respectively.
粒子凝集免疫分析（PACIA）是一种均质非放射免疫分析方法，已被应用于血清甲胎蛋白的测定。该技术基于包被有待测抗原抗体的乳胶颗粒的凝集反应。凝集反应通过使用一种设计用于血细胞计数的光学细胞计数器来测量，以确定未凝集颗粒数量的减少。通过用抗血清免疫球蛋白G组分的F(ab')2片段包被颗粒，可避免血清成分的干扰。该系统实现了自动化，采样率为每小时50次，孵育时间为26分钟。制备标准曲线所用的浓度范围为1至50微克/升；分析回收率为93.5%至98.4%；根据127份样本的结果计算，PACIA与放射免疫分析的相关系数为0.98；批内和批间变异系数的最大值分别为7.4%和9.6%。
