用单克隆抗体对2型副流感病毒血凝素-神经氨酸酶糖蛋白进行鉴定。

Parainfluenza virus type 2 haemagglutinin-neuraminidase glycoprotein characterized with monoclonal antibodies.

作者信息

Rydbeck R, Löve A, Orvell C

机构信息

Department of Virology, Karolinska Institute, School of Medicine, Stockholm, Sweden.

出版信息

J Gen Virol. 1988 Apr;69 ( Pt 4):931-5. doi: 10.1099/0022-1317-69-4-931.

DOI:10.1099/0022-1317-69-4-931

PMID:2451708

Abstract

Thirteen monoclonal antibodies (MAbs) were prepared against human parainfluenza virus type 2 (PIV2). These MAbs reacted with the haemagglutinin-neuraminidase glycoprotein with an Mr of 84K. The MAbs defined one antigenic site which could be divided into five epitopes. A correlation between haemagglutination inhibition (HI) and neutralization activity could be seen although one MAb, which recognized a distinct epitope, showed neutralization and no HI activity to PIV2. The reactivity of the MAbs was tested against Sendai virus, parainfluenza virus type 3, simian virus 5 (SV5), mumps virus, Newcastle disease virus, measles virus and canine distemper virus. Only one MAb showed any cross-reaction with a low HI titre to SV5.

摘要

制备了13种抗人2型副流感病毒（PIV2）的单克隆抗体（MAb）。这些单克隆抗体与分子量为84K的血凝素神经氨酸酶糖蛋白发生反应。这些单克隆抗体确定了一个抗原位点，该位点可分为五个表位。虽然有一种识别独特表位的单克隆抗体对PIV2表现出中和作用但无血凝抑制（HI）活性，但仍可观察到血凝抑制（HI）与中和活性之间的相关性。测试了这些单克隆抗体对仙台病毒、3型副流感病毒、猿猴病毒5（SV5）、腮腺炎病毒、新城疫病毒、麻疹病毒和犬瘟热病毒的反应性。只有一种单克隆抗体与SV5有低HI效价的交叉反应。