Nikitin V P, Lazetic B, Baic M, Sherstnev V V
Neirofiziologiia. 1987;19(5):637-45.
The possible role of S-100 brain specific proteins and their participation in the neuronal mechanisms of habituation were studied on identified neurons of the snail Helix pomatia. Extracellular application and intracellular injection of antibodies to S-100 proteins caused a rapid and significant decrease of neuronal responses to repeated stimulation in comparison with the control. During the action of antibodies a significant decrease of the evoked EPSP amplitude, increase of the action prepotential latency and a decrease in discharge frequency were observed. At the same time the membrane potential, membrane input impedance, pacemaker potentials did not change. The obtained effect is supposed to be caused by interruption of the synaptic transmission. Possible involvement of S-100 protein in the functioning of synaptic structures as one of the integrating apparatus of the neuron is discussed.
在罗马蜗牛已鉴定的神经元上，研究了S-100脑特异性蛋白的可能作用及其在习惯化神经元机制中的参与情况。与对照组相比，细胞外应用和细胞内注射S-100蛋白抗体导致神经元对重复刺激的反应迅速且显著降低。在抗体作用期间，观察到诱发的兴奋性突触后电位（EPSP）幅度显著降低、动作电位前潜伏期增加以及放电频率降低。同时，膜电位、膜输入阻抗、起搏电位没有变化。所获得的效应被认为是由突触传递中断引起的。讨论了S-100蛋白作为神经元整合装置之一可能参与突触结构功能的情况。
